FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458493
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT35790.24539027612748227No Hit
GACTTATAGTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGACATCA28110.19273318418394877No Hit
ATAGTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGA27250.18683668690902183No Hit
GTACAAACCAGGGAAAGCCCCTAGGCTCCTGATCCATTCTGCATCCACTT25910.17764912138762406No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24890.17065560136387353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24590.16859868370982925No Hit
ATTTATTACTGTGCGAGAGGGTTCGAGGGCCTATTCGGGAGGGCCTCCGA22410.15365174875710752No Hit
CTATAAGTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGATTGCAGACT21450.14706961226416582No Hit
GTAATAAATTGCCGTATCTTCCGATCTCAGGCTGCCCAGATTCATGTACA20450.14021322008401824No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17820.12218090865023007No Hit
GTGGGGTCCCTTCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACT16540.11340472665964114No Hit
GGGTACTATAAGTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGATTGC16390.112376267832619No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA16210.11114211724019243No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16020.10983940272596442No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15220.10435428898184632No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15190.1041485972164419No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15190.1041485972164419No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15090.10346295799842714No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT15080.10339439407662566No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29900.075.176241
TCAACGC34050.064.288054
ATCAACG34700.063.0837973
CAACGCA35350.061.9217225
TATCAAC38100.058.3972432
AACGCAG38000.057.599556
CGCAGAG42400.051.622248
ACGCAGA45000.048.6396187
GCAGAGT48750.044.750429
GGTATCA27350.034.5073131
ACATGGG41800.030.3186723
TACATGG41600.030.29032
CTAGACG1000.006281342428.8027364
GTGTAGA6200.027.8879571
GTACACG8900.026.698255
GTACATG57200.026.323731
TACACGG9400.025.2763966
CAGCGCT7650.023.5292269
CATGGGG25750.023.4896094
ATTGCCG11850.023.0884278
Produced by FastQC (version 0.11.5)