FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458493
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT33590.23030621333115756No Hit
GACTTATAGTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGACATCA25020.1715469323472927No Hit
ATAGTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGA24400.16729596919560122No Hit
GTACAAACCAGGGAAAGCCCCTAGGCTCCTGATCCATTCTGCATCCACTT22590.15488589934953406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22460.1539945683661149No Hit
GTAATAAATTGCCGTATCTTCCGATCTCAGGCTGCCCAGATTCATGTACA22430.15378887660071047No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21960.15056637227604108No Hit
ATTTATTACTGTGCGAGAGGGTTCGAGGGCCTATTCGGGAGGGCCTCCGA20900.14329859656508465No Hit
CTATAAGTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGATTGCAGACT19780.13561943732331935No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17700.12135814158861236No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16090.11031935017857475No Hit
GGGTACTATAAGTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGATTGC15640.10723397369750833No Hit
GTGGGGTCCCTTCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACT15620.10709684585390536No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)