FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750699
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15880.21153618161207088No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGA15310.2039432582166754No Hit
GTATATGGTACTACCACTACTACTGATGTATGAAACCCACTCCAGTCCCT14200.1891570389730105No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA13610.1812976972128643No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCCTCTTGGACGTTCGGCCAAG10860.1446651720596404No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10640.1417345700473825No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10240.13640620275236814No Hit
GTAGTAGAGGGTACCAATTGCTGCCCCACTCGCACAGTAATACACGGCCG10080.13427485583436236No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG9090.12108714677920179No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT8790.117090871307941No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8630.11495952438993526No Hit
TATAAAAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT8550.11389385093093238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC9800.048.5116164
GTATCAA11400.045.5062831
ATCAACG10900.044.27683
CAACGCA10800.044.0198025
TATCAAC11900.041.161372
AACGCAG11750.040.4337966
GGTATCA4600.039.158491
CCCGTTG3150.038.846148
CGCAGAG12550.037.853828
CCCCGTT3250.037.6508757
CCGTTGA3450.035.4682169
GTACATG17200.034.350191
TACATGG17600.030.6956272
ACATGGG17300.030.3951763
GCAGAGT15650.030.3556239
ATAGGTG8100.030.2358234
TAGGTGG8550.029.4869485
GGTACTA10050.029.3648177
GAATACG2502.6411726E-928.7918439
TCATAGG8800.028.6492522
Produced by FastQC (version 0.11.5)