FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746763
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG9700.12989395564590103No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC9510.12734964105077515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9360.1253409716335705No Hit
GATTATTACTGCACCTCATTTACAACCACCGGTACTGGTAGTTATGTCTT9300.12453750386668863No Hit
ACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACTACCAGTAC8310.1112802857131379No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8240.11034290665177574No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT8050.10779859205664984No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA7950.10645947911184672No Hit
GACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACTACCAGTA7700.10311169674983897No Hit
ATAATAGACGGCCGTGTCGTCAGATCTCAGGCTATTCAACTCCATGTAGA7660.10257605157191774No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC7510.10056738215471307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)