FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746763
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG10210.13672343166439688No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC9560.1280191975231767No Hit
GATTATTACTGCACCTCATTTACAACCACCGGTACTGGTAGTTATGTCTT9160.1226627457439643No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8220.1100750840628151No Hit
ACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACTACCAGTAC8030.10753076946768922No Hit
GACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACTACCAGTA7960.10659339040632705No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT7740.10364734192776022No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT7690.10297778545535867No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)