FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929148
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC30670.33008734884001256No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAGCACCACATGATCACTA24680.26561968599189795No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTAGTCCACTGCAGGTAGA23340.25119787159849666No Hit
CTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC21030.22633638559196168No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAGCACCAC20270.2181568490703311No Hit
CCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG18940.2038426601574776No Hit
ATGTATTACTGTGCGAAATCGGGCGATAGTACTGGGCAAGATGCTTATGA18610.20029101929940116No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT18130.19512499623310817No Hit
GTGTTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCAGAGCG17430.18759121259476422No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17240.1855463284643566No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16680.17951930155368143No Hit
CACTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCT16000.17220076887643304No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTT15930.17144739051259864No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15640.16832625157671327No Hit
GTAGTAGTGATCATGTGGTGCTCGGCGGAGGGACCAAGGTGACCGTCCTA15600.1678957496545222No Hit
GCCCTCAGGGATCCCAGAGCGAATCTCTGGCTCCAACTCTGGGAACACGG15480.16660424388794895No Hit
GCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCA15410.16585086552411454No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC14700.15820945640522285No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA14580.1569179506386496No Hit
CCACATGATCACTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCA14230.15315105881947763No Hit
TCTATTATGATAGCGACCGGCCCTCAGGGATCCCAGAGCGAATCTCTGGC13950.15013754536414006No Hit
TGGTAGTAGTGATCATGTGGTGCTCGGCGGAGGGACCAAGGTGACCGTCC13790.1484155376753757No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13720.14766215931154134No Hit
CTATTATGATAGCGACCGGCCCTCAGGGATCCCAGAGCGAATCTCTGGCT13220.14228088528415278No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAGCACCACATGATCACTAC13210.142173259803605No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA13170.14174275788141394No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13150.1415275069203184No Hit
CTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCC13130.14131225595922287No Hit
CCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCAC12880.1386216189455286No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA12320.13259459203485344No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT12250.13184121367101903No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA12100.13022683146280248No Hit
GACCAACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTT12100.13022683146280248No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA12090.1301192059822547No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12090.1301192059822547No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11920.12828957281294262No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11790.1268904415658216No Hit
ATCATGTGGTGCTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCC11610.12495318291596172No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT11470.12344642618829293No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11410.12280067330500631No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11410.12280067330500631No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11410.12280067330500631No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11400.12269304782445854No Hit
CCCCTGTGTTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCA11380.12247779686336299No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11340.12204729494117192No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11000.11838802860254771No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG10990.11828040312199994No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10910.11741939927761777No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG10830.11655839543323562No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT10670.11483638774447127No Hit
GTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACGGCCAG10620.11429826034173242No Hit
GGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTAC10230.11010086660036937No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC10150.1092398627559872No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGTTGGTCATCTATTATGAT10110.10880936083379612No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT10050.1081636079505095No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC9970.10730260410612733No Hit
GACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGC9930.10687210218393625No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG9790.10536534545626747No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9780.1052577199757197No Hit
GTCTGGAGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAAGATCTCCTGTA9370.1008450752732611No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)