FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929148
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC32810.3531192016772355No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTAGTCCACTGCAGGTAGA23410.2519512499623311No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAGCACCACATGATCACTA21380.23010327741113365No Hit
CTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC20210.21751109618704448No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19830.2134213279262292No Hit
CCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG19810.21320607696513363No Hit
ATGTATTACTGTGCGAAATCGGGCGATAGTACTGGGCAAGATGCTTATGA18420.19824613516899353No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAGCACCAC17700.19049710056955405No Hit
GTGTTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCAGAGCG17560.18899034384188526No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17230.18543870298380882No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTT16250.1748914058901273No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16160.17392277656519736No Hit
CACTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCT15690.16886437897945217No Hit
GTAGTAGTGATCATGTGGTGCTCGGCGGAGGGACCAAGGTGACCGTCCTA15690.16886437897945217No Hit
GCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCA15610.16800337513507No Hit
GCCCTCAGGGATCCCAGAGCGAATCTCTGGCTCCAACTCTGGGAACACGG15480.16660424388794895No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14440.15541119391098082No Hit
TCTATTATGATAGCGACCGGCCCTCAGGGATCCCAGAGCGAATCTCTGGC14100.15175192757235662No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13990.15056804728633114No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT13830.14884603959756681No Hit
TGGTAGTAGTGATCATGTGGTGCTCGGCGGAGGGACCAAGGTGACCGTCC13580.14615540258387252No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA13470.14497152229784707No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC13370.14389526749236936No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13100.14098937951757953No Hit
CTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCC13070.14066650307593623No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12910.1389444953871719No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12710.13679198577621648No Hit
CTATTATGATAGCGACCGGCCCTCAGGGATCCCAGAGCGAATCTCTGGCT12700.13668436029566872No Hit
CCACATGATCACTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCA12690.13657673481512095No Hit
CCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCAC12610.1357157309707388No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12000.12915057665732477No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11990.12904295117677703No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11940.12850482377403813No Hit
GACCAACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTT11770.12667519060472604No Hit
CCCCTGTGTTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCA11550.1243074300326751No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11540.12419980455212731No Hit
ATCATGTGGTGCTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCC11490.12366167714938847No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA11450.1232311752271974No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11180.1203252872524076No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11110.11957190888857319No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG11100.11946428340802542No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT11020.11860327956364325No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGTTGGTCATCTATTATGAT10940.11774227571926109No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10910.11741939927761777No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAGCACCACATGATCACTAC10800.11623551899159229No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10750.11569739158885345No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10560.1136525074584458No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG10460.1125762526529681No Hit
GGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTAC10410.11203812525022924No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG10270.11053136852256044No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC9970.10730260410612733No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9940.10697972766448402No Hit
GTCTGGAGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAAGATCTCCTGTA9910.10665685122284072No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9910.10665685122284072No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT9820.10568822189791077No Hit
GTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACGGCCAG9740.1048272180535286No Hit
GACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGC9590.10321283584531205No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT9460.10181370459819103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)