FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107615
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTGCGAAAGATCAGTATCATGACTATGATTCGGAGACTTA53860.4862700487082605No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACA53570.48365181042149125No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28320.25568451131485215No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27990.25270513671266637No Hit
GCCGTATATTACTGTGCGAAAGATCAGTATCATGACTATGATTCGGAGAC27650.24963547803162653No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC27170.24530184224662901No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26010.23482888909955174No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA25120.22679360608153556No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24260.2190291753000817No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23880.21559838030362535No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACACCG21130.19077025861874386No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20730.18715889546457928No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG19590.17686651047521024No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19060.17208145429594218No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18890.17054662495542225No Hit
GTGTATCTGCAAATGAGCAGCCTGAGAGCCGAGGACACGGCCGTATATTA18550.16747696627438233No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTTAGAAGCTTTGCCATGAGCTGGG17940.16196963746428136No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA17560.15853884246782501No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17490.15790685391584622No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16500.14896873010928888No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16390.14797560524189363No Hit
ATATTACTGTGCGAAAGATCAGTATCATGACTATGATTCGGAGACTTATT16020.1446350943242914No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACTTTTAGAAGCTTTGCC15840.14300998090491732No Hit
GTATTACTGTCAACAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGA15660.14138486748554327No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA15570.14057231077585622No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15450.13948890182960685No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15370.13876662919877394No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT14840.1339815730195059No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT14770.13334958446752707No Hit
TACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCAT14640.1321758914424236No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC14250.12865481236711312No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC14230.12847424420940487No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG13970.1261268581591979No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC13620.1229669153993039No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGTTGTTGGAGTCTGGGGGAGGCT13620.1229669153993039No Hit
GTGTGAGGTGCAGTTGTTGGAGTCTGGGGGAGGCTTGGTGCGGCCTGGGG13600.12278634724159569No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13410.12107094974336752No Hit
GTATCATGACTATGATTCGGAGACTTATTATAATTGGTTCGACCCCTGGG13400.1209806656645134No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG13400.1209806656645134No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG13380.12080009750680516No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT13340.12043896119138871No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13130.11854299553545229No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13050.11782072290461938No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13050.11782072290461938No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC13010.11745958658920294No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT12920.11664702987951589No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12900.11646646172180768No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC12820.11574418909097477No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT12770.11529276869670418No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12600.11375793935618422No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA12550.11330651896191365No Hit
GTATGTGCGTCCACCACTCCCACTAATTGCTGAGACCCAATGCAGCCCCT12390.11186197370024784No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC12200.11014657620201966No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC11560.10436839515535633No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT11300.10202100910514936No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15050.077.065031
GGTATCA5900.067.953971
TCAACGC18350.062.3635524
TATCAAC19200.060.38592
ATCAACG18850.060.3490033
CAACGCA19150.059.38485
AACGCAG20400.056.431936
CGCAGAG24000.047.967148
GCAGAGT27600.041.68429
CCGATCG908.148955E-539.6992389
TATACGG24150.037.9087755
ATACGGC24850.036.5431986
GTAATAT25900.036.488251
TACGGCC25300.035.893227
TACATGG25550.035.013932
GTACATG26000.034.6957361
AATATAC27050.034.648813
TAATATA27600.034.22842
ACATGGG26400.033.875873
ATATACG28750.032.5897034
Produced by FastQC (version 0.11.5)