FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107615
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTGCGAAAGATCAGTATCATGACTATGATTCGGAGACTTA49610.4478993151952619No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACA49520.4470867584855749No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30170.2723870659028633No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25240.22787701502778496No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC24290.21930002753664407No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24110.21767491411726997No Hit
GCCGTATATTACTGTGCGAAAGATCAGTATCATGACTATGATTCGGAGAC23940.21614008477675004No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA22210.20052093913498825No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21910.19781241676936479No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21430.1934787809843673No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACACCG20640.18634633875489226No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19050.17199117021708807No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT18010.16260162601626016No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA17370.15682344496959683No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17260.15583032010220157No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17010.15357321813084873No Hit
GTGTATCTGCAAATGAGCAGCCTGAGAGCCGAGGACACGGCCGTATATTA16890.15248980918459934No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTTAGAAGCTTTGCCATGAGCTGGG16780.1514966843172041No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16770.15140640023834998No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16740.1511355480017876No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA16640.1502327072132465No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16200.14626020774366544No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT16040.1448156624819996No Hit
GTATTACTGTCAACAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGA15950.14400310577231257No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT15600.1408431630124186No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACTTTTAGAAGCTTTGCC14230.12847424420940487No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC14070.12702969894773905No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT13940.12585600592263557No Hit
ATATTACTGTGCGAAAGATCAGTATCATGACTATGATTCGGAGACTTATT13640.12314748355701213No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT13630.12305719947815802No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC13060.1179110069834735No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG12890.11637617764295356No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12810.11565390501212063No Hit
GTATCATGACTATGATTCGGAGACTTATTATAATTGGTTCGACCCCTGGG12800.11556362093326651No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC12780.1153830527755583No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12670.11438992790816303No Hit
GTGTGAGGTGCAGTTGTTGGAGTCTGGGGGAGGCTTGGTGCGGCCTGGGG12470.11258424633108075No Hit
GTATGTGCGTCCACCACTCCCACTAATTGCTGAGACCCAATGCAGCCCCT12430.11222311001566429No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC12410.11204254185795605No Hit
TACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCAT12240.11050771251743612No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA12120.10942430357118674No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC11910.10752833791525035No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11830.10680606528441741No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11820.1067157812055633No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG11630.10500038370733512No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT11490.10373640660337753No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT11410.10301413397254462No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGTTGTTGGAGTCTGGGGGAGGCT11400.10292384989369051No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT11270.10175015686858702No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12550.069.433371
ATCAACG14350.057.7128523
TCAACGC14350.057.7128524
TATAACG502.0780634E-457.6124842
TATCAAC14800.056.444662
CAACGCA15100.054.8463175
GTACCGA400.00621366954.01171
AACGCAG16450.050.7646986
GGTATCA5850.046.7793661
CGCAGAG18450.045.2617538
GGGCAGT7650.037.6552161
GCAGAGT22350.037.3637289
ACATGGG26950.035.5401653
GTACATG27700.035.097861
GAATACG2501.8189894E-1234.5550089
TACATGG27700.034.3179052
CATGGGA15500.033.9170274
TATACGG23000.033.502915
TACGGCC23250.033.1306957
ATACGGC23050.033.1058436
Produced by FastQC (version 0.11.5)