FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622879
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20540.3297590703812458No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCACCTGGTGGGGTCTGGGGGAGGCG18170.29170994687571744No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17890.2872146917780179No Hit
ATTTATTACTGTGAGGCAGAGGGGGCTACGGTTTCGTCCTACTTTGACTA17800.2857697883537573No Hit
GTAATAAATAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGCAGATACA17480.2806323539563864No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16880.27099966446131596No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16810.269875850686891No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT16550.26570168523902715No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16060.25783498881805295No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15450.24804175449806465No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12780.20517628624500103No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG12600.2022864793964799No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG12530.201162665622055No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA12070.1937776036758343No Hit
TTATTACTGTGAGGCAGAGGGGGCTACGGTTTCGTCCTACTTTGACTACT11630.1867136313794493No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11590.18607145207977793No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11310.18157619698207839No Hit
GGGCTGGAGTGGGTGGCAGTTACATCACCTGGTGGAAGGGATAACTACTA10530.16905370063848676No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10380.16664552826471915No Hit
CAGTAATAAATAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGCAGATA10290.16520062484045858No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT10220.1640768110660337No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACGTTTGCTATGCACTGGG10080.16182918351718392No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9830.15781556289423787No Hit
GTTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGAATTGTCTCT9680.15540739052047026No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9640.1547652112207989No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG9080.1457747010253998No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC8870.1424032597021251No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC8780.14095835627786457No Hit
GTATCTGCAAATGAACAGCCTGAGAACTGAGGACACGGCTATTTATTACT8730.14015563215327537No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA8640.1387107287290148No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG8530.13694473565491855No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8440.13549983223065798No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC8290.13309165985689034No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8090.12988076335853352No Hit
CTGTATCTGCAAATGAACAGCCTGAGAACTGAGGACACGGCTATTTATTA8030.12891749440902647No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT8010.1285964047591908No Hit
GGATAACTACTACGCGGACTCCGTGAAGGGCCGATTCACCATCTCCAGAG7970.12795422545951943No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7960.1277936806346016No Hit
CTACTACGCGGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT7940.1274725909847659No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTACGTTTGCT7920.12715150133493022No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT7790.1250644186109983No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT7750.12442223931132693No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT7660.12297733588706634No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC7580.12169297728772362No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT7530.12089025316313441No Hit
CTCCAGAGACAATTCCAAGAACACACTGTATCTGCAAATGAACAGCCTGA7500.12040861868838088No Hit
GTCCAGTGTCAGGTGCACCTGGTGGGGTCTGGGGGAGGCGTGGTCCAGCC7410.11896371526412032No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC7340.11783990148969542No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG7310.11735826701494191No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG7310.11735826701494191No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC7240.11623445324051701No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC7010.11254192226740668No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCAAACGTAC6910.11093647401822825No Hit
GCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA6910.11093647401822825No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA6910.11093647401822825No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT6760.10852830164446063No Hit
GTGATGGAAACACCTACTTGAATTGGCTTCAGCAGAGGCCAGGCCAGCCT6740.10820721199462496No Hit
GTGTCAGGTGCACCTGGTGGGGTCTGGGGGAGGCGTGGTCCAGCCTGGGA6730.10804666716970712No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG6700.10756503269495359No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC6680.10724394304511792No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG6670.10708339822020008No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC6660.10692285339528222No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT6640.10660176374544655No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG6600.10595958444577518No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA6540.10499631549626813No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG6510.10451468102151461No Hit
GCCTGAGAACTGAGGACACGGCTATTTATTACTGTGAGGCAGAGGGGGCT6350.10194596382282915No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT6330.10162487417299346No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA6240.1001799707487329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2300.075.13111
GTATCAA6350.068.03211
TCAACGC6800.063.529984
ATCAACG7000.061.7148363
CAACGCA7450.057.987095
TATCAAC8050.054.5594942
AACGCAG8350.052.5908286
CGCAGAG9500.045.466798
GGCAGCG5450.042.268827
CAGCGCT5750.041.315479
GCAGAGT11350.038.690179
AGTACCG750.001534184138.4003455
GCAGCGC6000.038.394188
TCTATAG1001.4485519E-436.000323
CAGGGCA6700.035.4634
GTACATG14000.030.8574181
ACGCAGA14350.030.6016317
TACATGG13450.030.5132832
GTACACG9450.028.9526395
GGCTTAG1000.00628125828.8002551
Produced by FastQC (version 0.11.5)