FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641886
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCCGTGTCCTCAGATCTCAGGCTACTCAGCTCCATGTAGG15260.23773691901677244No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC14080.219353592382448No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAAGTCTCCTGCA12720.1981660294818706No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11320.1763553029665704No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT10710.16685205784204674No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC10560.16451519428683598No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT10530.16404782157579384No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10160.15828355813960734No Hit
GTTTATTACTGTGCGAGAGTATCGGGAATGACAGCAGCTGGTAGGTTACC10010.1559466945843966No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGTTGCTG9910.15438878554758945No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC9770.15220771289605944No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGTTGCTGC9720.15142875837765585No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9450.14722240397827652No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA9310.1450413313267465No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9300.14488554042306578No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9220.14363921319362005No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT9150.14254867686785505No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT9010.14036760421632502No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8660.13491492258749996No Hit
GGTTATGACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA8540.1330454317433314No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT8500.13242226812860852No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT8470.13195489541756636No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG8250.1285274955365906No Hit
GATTATTACTGCAGCTCATATGCGGGCAGCAACAATTATGTCTTCGGAAC8140.12681379559610273No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGCTGGTTATGACTATGTCTCCT7990.12447693204089201No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7970.12416535023353056No Hit
CTACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCT7900.12307481390776555No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7790.1213611139672777No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA7770.12104953215991625No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7700.11995899583415123No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC7580.11808950498998264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.11684317776053692No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG7470.11637580504949477No Hit
GCCTACATGGAGCTGAGTAGCCTGAGATCTGAGGACACGGCCGTTTATTA7320.11403894149428404No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG6970.10858625986545899No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6910.10765151444337467No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT6840.10656097811760966No Hit
GCGTAGTAGTAGTAGTTCGGTAACCTACCAGCTGCTGTCATTCCCGATAC6760.10531465088816393No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6700.10437990546607964No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6610.10297778733295321No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG6520.10157566919982676No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT6520.10157566919982676No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6510.10141987829614604No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA6420.10001776016301961No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3500.073.651241
GTATCAA10150.069.841691
TCAACGC12500.056.0598534
ATCAACG12650.054.2815173
CAACGCA13150.053.288835
TATCAAC13800.050.2881362
AACGCAG14150.049.0060426
CGCAGAG16000.043.3397188
GCAGAGT18550.037.370329
ACGCAGA19500.035.5607957
CAGAGTA17500.033.4868859
CCGTGCA1102.6494215E-432.484519
GTACATG15950.032.3234251
ATACGCT900.00388565831.772538
ACATGGG16200.031.3483453
GTAATAG2152.0405423E-829.974341
GTACACG5600.028.0912155
GAATACG2304.005051E-827.9649249
TATAGGA2156.8708505E-726.6210022
GTGCTAG2809.340511E-925.5733471
Produced by FastQC (version 0.11.5)