FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641886
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCCGTGTCCTCAGATCTCAGGCTACTCAGCTCCATGTAGG15240.237425337209411No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAAGTCTCCTGCA12190.18990911158679266No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC11400.1776016301960161No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC11020.1716815758561489No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10600.16513835790155884No Hit
GTTTATTACTGTGCGAGAGTATCGGGAATGACAGCAGCTGGTAGGTTACC10540.16420361247947454No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT10420.16233412163530594No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10330.1609320035021795No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGTTGCTGC10060.15672564910280018No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGTTGCTG9950.1550119491623123No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT9700.15111717657029441No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT9550.14878031301508368No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA9260.14426237680834292No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC9200.14332763138625862No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9090.14161393144577072No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT9060.1411465587347286No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8850.13787494975743356No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8790.13694020433534926No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8680.1352265043948614No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8020.12494430475193413No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC7840.12214006848568126No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7810.12167269577463911No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG7780.12120532306359695No Hit
GGTTATGACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA7650.11918004131574765No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT7600.11840108679734408No Hit
GATTATTACTGCAGCTCATATGCGGGCAGCAACAATTATGTCTTCGGAAC7480.1165315959531755No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT7430.1157526414347719No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC7300.1137273596869226No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7290.11357156878324189No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7250.11294840516851902No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG7080.11029995980594687No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGCTGGTTATGACTATGTCTCCT6990.1088978416728204No Hit
CTACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCT6970.10858625986545899No Hit
GCCTACATGGAGCTGAGTAGCCTGAGATCTGAGGACACGGCCGTTTATTA6550.10204304191086891No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)