FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401004
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATATACACTGGGTGCGACAGGCCCCTGGACGAGGGCTTGAGTGGTTG15830.39475915452214944No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10890.2715683634078463No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9900.2468803303707694No Hit
CTACTATATACACTGGGTGCGACAGGCCCCTGGACGAGGGCTTGAGTGGT8000.1994992568652682No Hit
GTTTACGTCTCTCGCACAGAAATACACGGCGGTGTCCTCAGATGTCAGGC6820.17007311647764112No Hit
CTCCTGATCCATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT6780.1690756201933148No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACGAGGGCTTGAGTGGTTGG6380.15910065735005136No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6270.15635754256815393No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC6010.14987381672003272No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5950.1483775722935432No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5810.14488633529840103No Hit
GACGTAAACAGTGCGAGCCAAAGGGGGGCTTATGACTACTGGGGCCAGGG5750.1433900908719115No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5560.13865198352136138No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5480.13665699095270872No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5350.13341512802864808No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG5180.12917576882026113No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5120.12767952439377164No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA5110.12743015032269006No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG5050.12593390589620054No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT5040.12568453182511893No Hit
GAAATACACGGCGGTGTCCTCAGATGTCAGGCTGCTCAGCTGCATATAAA4690.11695643933726348No Hit
GACTTACAGTTCCCCGTGGACATTCGGCCAAGGGACCAAGGCGGAAATCA4660.11620831712401872No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT4600.1147120726975292No Hit
GTCTCCATCCTCCCTGTCTGCTTCTGTTGGAGACAGAGTCACCATCACTT4580.11421332455536604No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT4570.11396395048428444No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAAGGAATCAGTAA4330.10797897277832641No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC4290.10698147649400006No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG4270.10648272835183689No Hit
GTGTATTTCTGTGCGAGAGACGTAAACAGTGCGAGCCAAAGGGGGGCTTA4250.10598398020967373No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG4220.10523585799642897No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4100.10224336914344993No Hit
TCTGAGGACACCGCCGTGTATTTCTGTGCGAGAGACGTAAACAGTGCGAG4090.10199399507236835No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC4080.10174462100128677No Hit
GTCAAGGAATCAGTAACTTTTTAAATTGGTATCAACAGAAACCAGGGAAA4070.10149524693020519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG8950.060.959683
CAACGCA8900.060.5162165
TCAACGC8900.060.5162164
GAATACG1052.3646862E-1159.9548389
AACGCAG9650.056.5377246
ACGCAGA11050.048.741577
GTGCTAG1600.048.088781
CGCAGAG11500.046.8342938
ATAGTAC856.5918604E-541.145483
GCAGAGT13100.040.0461739
GATTCAC1401.9357685E-839.9698941
GTACATG15150.039.706071
GTACTGG2850.039.268671
TACATGG14950.038.3657532
GTATCAA15150.037.3975751
GTCCTAC750.001767729437.3052331
GAGTTTA1753.43789E-935.9729081
TATCAAC15800.035.4163632
GCTAGAG1855.9153535E-934.0284273
ACATGGG16850.033.6245273
Produced by FastQC (version 0.11.5)