FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448586
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11160.2487817274725471No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9600.2140057870731588No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9520.2122224055142158No Hit
GTCTATTTCTGTGCGAGACGGTCGAAATTATTATGGTTTGGGGAGTTTAC9390.20932441048093342No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT9350.20843271970146193No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8870.197732430347804No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8650.19282813106071078No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8130.18123615092758133No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7940.17700061972509173No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7810.17410262469180937No Hit
CTCTTATCGTGGGACCACTTACTTCAATCCGTCCCTCAAGAGTCGAGTCA7740.17254216582773427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7400.16496279420222656No Hit
CCCTGAGACTGAGCTCTGTGACCGCCGCAGACACGGCTGTCTATTTCTGT6420.14311637010517492No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT5810.12951808571823464No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA5750.12818054954902738No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5700.12706593607468802No Hit
AGTTACTACTGGGCCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTG5650.12595132260034864No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC5620.12528255451574505No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT5510.12283040487219843No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG5400.1203782552286518No Hit
GTCTCTGGTGGCTCCATCATCGGTAGTAGTTACTACTGGGCCTGGATCCG5390.12015533253378394No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT5350.11926364175431244No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5260.11725733750050159No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC5050.11257596090827623No Hit
GTCTCCAGCCACTCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCT5040.11235303821340835No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG5010.11168427012880475No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA5000.11146134743393685No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA4960.11056965665446536No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC4930.10990088856986174No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4880.10878627509552238No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT4710.10499658928276852No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4680.10432782119816489No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC4640.1034361304186934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4630.10321320772382553No Hit
GTATACTAACTGGCCCCTTTTCGGCCCTGGGACCAAAGTGCATTTCAAAC4600.10254443963922191No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA4590.10232151694435404No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT4540.10120690347001467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6300.089.959911
GTATCAA14850.073.5025941
TATCAAC19250.056.338532
ATCAACG19300.056.192573
TCAACGC19300.056.192574
CAACGCA19500.055.2574235
AACGCAG20250.053.2108576
GAATACG851.1388929E-649.3897559
CGCAGAG23500.045.8519068
ACGCAGA25000.043.1007927
GCAGAGT25600.042.090629
CCTATAC1507.585186E-1041.981293
GACCGTT856.581182E-541.158137
GTGCTAG1651.9299478E-938.164811
CTATAGG750.001765287137.31671
ATACGGC800.00242315434.984416
GTACATG15650.034.425551
TCCCGTG5850.033.331303145
ACATGGG15850.033.108273
TACATGG15900.033.0041582
Produced by FastQC (version 0.11.5)