FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448586
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9950.22180808139353436No Hit
GTCTATTTCTGTGCGAGACGGTCGAAATTATTATGGTTTGGGGAGTTTAC9140.20375134310923657No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT9010.20085334807595423No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8500.18948429063769265No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8490.18926136794282478No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8390.18703214099414606No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7820.17432554738667724No Hit
CTCTTATCGTGGGACCACTTACTTCAATCCGTCCCTCAAGAGTCGAGTCA7560.16852955732011254No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA7390.16473987150735867No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7100.15827511335619035No Hit
CCCTGAGACTGAGCTCTGTGACCGCCGCAGACACGGCTGTCTATTTCTGT6930.15448542754343647No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT6340.14133298854623194No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6200.1382120708180817No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA5910.13174731266691336No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT5820.1297410084131025No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT5760.12840347224389528No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG5570.12416794104140566No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC5440.12126994600812331No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT5440.12126994600812331No Hit
GTCTCTGGTGGCTCCATCATCGGTAGTAGTTACTACTGGGCCTGGATCCG5400.1203782552286518No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5380.11993240983891607No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC5370.1197094871440482No Hit
GTATACTAACTGGCCCCTTTTCGGCCCTGGGACCAAAGTGCATTTCAAAC5280.11770318289023732No Hit
AGTTACTACTGGGCCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTG5240.11681149211076583No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT5110.11391349707748347No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC4960.11056965665446536No Hit
GTCTCCAGCCACTCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCT4880.10878627509552238No Hit
CATTTATGGTGCATCTACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTG4770.10633412545197576No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC4730.10544243467250428No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC4640.1034361304186934No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC4580.10209859424948615No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG4520.10076105808027892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)