FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324246
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAACTCTCAGGCTGTTCATCTGCAGATACA70800.5346438652637048No Hit
GTGTATTACTGTGCGAGATCAGGTATTAGCAGCTCCGGGGGTATGGACGT60660.45807198964542845No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA38870.2935255232033927No Hit
TTTAAGAGGTGTCCAGAGTCAGGTGCAGCTGGTGGAGTCAGGGGGAGGCG37820.28559648282871913No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC35960.2715507541650117No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35740.26988943141984195No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT34770.26256450840704826No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC33480.25282311594673496No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA32050.24202451810313189No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC31890.24081628337937214No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG28610.2160474715422965No Hit
GTTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGAC28270.21347997275430697No Hit
GTATTACTGTGCGAGATCAGGTATTAGCAGCTCCGGGGGTATGGACGTCT26850.2027568895809389No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG26560.20056696414412428No Hit
GGTATTAGCAGCTCCGGGGGTATGGACGTCTGGGGCCAAGGGACCACGGT26010.1964136572812No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTCAGTAGTTATGTTATGCACTGGG25250.19067454234334102No Hit
CAGTTATATCAGATGATGGCAGTCAGACACAGAACGCAGACTCCGTGAAG24810.18735189685300163No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT24100.1819903552663176No Hit
GTCCAGAGTCAGGTGCAGCTGGTGGAGTCAGGGGGAGGCGTTGTCCAGCC23060.17413682956187898No Hit
GGGCTGGAGTGGGTGACAGTTATATCAGATGATGGCAGTCAGACACAGAA21850.1649995544634456No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC21760.16431992243133076No Hit
ATACACAGCCGTGTCCTCAACTCTCAGGCTGTTCATCTGCAGATACAACG21490.1622810263349861No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT21390.16152587963263623No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21380.16145036496240125No Hit
CAGTAATACACAGCCGTGTCCTCAACTCTCAGGCTGTTCATCTGCAGATA21080.15918492485535166No Hit
GTCTCTGGATTCACCTTCAGTAGTTATGTTATGCACTGGGTCCGCCAGGC20940.15812771947206183No Hit
GCTAATACCTGATCTCGCACAGTAATACACAGCCGTGTCCTCAACTCTCA20400.15404992727937256No Hit
CTCCAGAGACAACTCCAAGAACACGTTGTATCTGCAGATGAACAGCCTGA19890.15019867909738824No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA19880.15012316442715326No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA19690.14868838569268852No Hit
GCTGTGTATTACTGTGCGAGATCAGGTATTAGCAGCTCCGGGGGTATGGA19430.14672500426657886No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG18920.14287375608459454No Hit
TCACTGAACACAGAGGACGCACCATGGAGTTTGGGCTGAGCTGGGTTTTC18830.1421941240524797No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC18720.14136346267989483No Hit
GTAGTTATGTTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAG18580.140306257296605No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG18550.14007971328590005No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT17980.13577537708250584No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT17670.13343442230522123No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT17650.13328339296475128No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC17630.1331323636242813No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG17610.13298133428381131No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA17470.1319241289005215No Hit
CCCTGAGACTCTCCTGTGCAGTCTCTGGATTCACCTTCAGTAGTTATGTT16880.1274687633566573No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC16720.1262605286328975No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC16710.12618501396266252No Hit
GCCTGAGAGTTGAGGACACGGCTGTGTATTACTGTGCGAGATCAGGTATT16570.12512780857937272No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16450.12422163253655288No Hit
GCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGTCTCTGGATTCACCTTCA16370.123617515174673No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT16340.12339097116396802No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG16340.12339097116396802No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16250.12271133913185313No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC16190.12225825111044322No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15620.117953914907049No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA15590.11772737089634404No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT15350.11591501881070435No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC15240.11508435743811951No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA14600.11025141854308038No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC14130.106702229042036No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14050.1060981116801561No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA13910.10504090629686628No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG13800.10421024492428144No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC13630.10292649553028668No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCT13410.10126517278511696No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG13390.10111414344464698No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC13350.10081208476370705No Hit
GAACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACTCCA13350.10081208476370705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18300.075.705061
GGTATCA7000.072.156391
TCAACGC21350.064.444734
ATCAACG21750.062.9283453
CAACGCA21850.062.6379745
TATCAAC23500.059.1618082
CGCAGAG27900.049.268698
CAGCGCT9200.044.5891159
GCAGAGT32100.042.5981189
GCAGCGC9700.042.2907078
GTCGTAT750.001518599138.4834061
GGCAGCG10450.037.189417
GTACATG29300.032.999851
TACATGG29900.032.5247962
CAGCCGT38550.031.7370349
ACATGGG30250.031.67223
GTAATAC39900.031.2858521
GTATGTA21000.030.9241681
GTACACG19950.029.9688955
AATACAC42850.029.2516523
Produced by FastQC (version 0.11.5)