FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324246
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAACTCTCAGGCTGTTCATCTGCAGATACA66720.5038338798078303No Hit
GTGTATTACTGTGCGAGATCAGGTATTAGCAGCTCCGGGGGTATGGACGT55800.42137185991122494No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT36460.27532648767676093No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA35130.2652830365355077No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC33360.25191693990391517No Hit
TTTAAGAGGTGTCCAGAGTCAGGTGCAGCTGGTGGAGTCAGGGGGAGGCG32910.24851877974334075No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC31880.2407407687091371No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT30090.22722364273707454No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA30090.22722364273707454No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC27350.20653262309268824No Hit
GTATTACTGTGCGAGATCAGGTATTAGCAGCTCCGGGGGTATGGACGTCT24770.1870498381720617No Hit
GTTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGAC23820.17987594449973798No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG23550.17783704840339332No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT23430.17693087236057348No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22990.17360822687023408No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTCAGTAGTTATGTTATGCACTGGG22710.17149381610365447No Hit
GGTATTAGCAGCTCCGGGGGTATGGACGTCTGGGGCCAAGGGACCACGGT22150.1672649945704952No Hit
GTCCAGAGTCAGGTGCAGCTGGTGGAGTCAGGGGGAGGCGTTGTCCAGCC21800.16462198111227067No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA21550.162734114356396No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21130.15956249820652657No Hit
CAGTTATATCAGATGATGGCAGTCAGACACAGAACGCAGACTCCGTGAAG20550.15518264733289736No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT20210.15261514854490782No Hit
GTCTCTGGATTCACCTTCAGTAGTTATGTTATGCACTGGGTCCGCCAGGC20100.15178448717232296No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT19790.14944353239503838No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19390.14642294558563893No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC19260.1454412548725841No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18980.14332684410600446No Hit
GCTAATACCTGATCTCGCACAGTAATACACAGCCGTGTCCTCAACTCTCA18830.1421941240524797No Hit
CAGTAATACACAGCCGTGTCCTCAACTCTCAGGCTGTTCATCTGCAGATA18600.140457286637075No Hit
GGGCTGGAGTGGGTGACAGTTATATCAGATGATGGCAGTCAGACACAGAA18220.1375877291681455No Hit
GCTGTGTATTACTGTGCGAGATCAGGTATTAGCAGCTCCGGGGGTATGGA18060.1363794944443857No Hit
CTCCAGAGACAACTCCAAGAACACGTTGTATCTGCAGATGAACAGCCTGA18000.1359264064229758No Hit
ATACACAGCCGTGTCCTCAACTCTCAGGCTGTTCATCTGCAGATACAACG17940.13547331840156587No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG17490.13207515824099147No Hit
GTAGTTATGTTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAG17410.1314710408791116No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC17400.1313955262088766No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC16770.12663810198407244No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16330.12331545649373304No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT16110.12165413374856335No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG16020.12097450171644845No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT15880.11991729633315865No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA15530.11727428287493412No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA15520.11719876820469914No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG15330.11576398947023439No Hit
GCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGTCTCTGGATTCACCTTCA15170.1145557547464746No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC15170.1145557547464746No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15140.11432921073576965No Hit
GCCTGAGAGTTGAGGACACGGCTGTGTATTACTGTGCGAGATCAGGTATT14750.11138413859660516No Hit
CCCTGAGACTCTCCTGTGCAGTCTCTGGATTCACCTTCAGTAGTTATGTT14630.11047796255378532No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA14580.1101003892026104No Hit
TCACTGAACACAGAGGACGCACCATGGAGTTTGGGCTGAGCTGGGTTTTC14500.10949627184073049No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT14210.10730634640391588No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC13800.10421024492428144No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13730.10368164223263654No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC13390.10111414344464698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13700.065.6800461
ATCAACG13900.063.1073153
TCAACGC13800.063.0459754
CAACGCA13800.062.0062375
TATCAAC15800.055.063392
CGCAGAG18150.047.152378
GCAGAGT21700.039.4385039
GTACATG27200.039.433141
CAGCGCT8900.037.9787869
TACATGG28400.036.9631652
ACATGGG28700.036.075743
GGCAGCG10050.035.7770357
GCAGCGC9800.035.2247858
GGTATCA6150.035.1147961
GTATGTA19250.033.281531
CAGCCGT34300.032.708739
GTACACG18250.031.5206135
GTAATAC35150.031.3335861
AATACAC37850.030.3940643
TACACGG18950.030.3585576
Produced by FastQC (version 0.11.5)