FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130926
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGACTATGCGGACTCTGTGAAGGGCCGATTCACCATGTCCAGAGA42840.3788046255900032No Hit
GTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGGAGGTACA31250.27632223505339876No Hit
GTCTATGACAGCACTGTTCCAATTAATACCTGAGACCCATTCCAGGCCCT26240.23202225432963783No Hit
GTCCAGAGACAGCGCCAAGAACTCCGTGTACCTCCAAATGAACAGTCTGA25290.22362205838401453No Hit
TTGTATTACTGTGCAAAAGATTTCAGCGGCTTTTCGAGGGGTTTATTCGA24500.21663663228186458No Hit
CTCATGTACAGTCTCTGGATTCACCCTTGAGGATCATGCCATGCACTGGG23270.20576058911016284No Hit
ATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGGAGGTACACGG22840.20195839515582806No Hit
GCCTTGTATTACTGTGCAAAAGATTTCAGCGGCTTTTCGAGGGGTTTATT21750.1923202755971655No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21450.18966758214065288No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC20120.1779073078167802No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19020.1681807651429006No Hit
GTCTCTGGACATGGTGAATCGGCCCTTCACAGAGTCCGCATAGTCTATGA18770.16597018726247342No Hit
GTCTGAGAGCTGAGGACACGGCCTTGTATTACTGTGCAAAAGATTTCAGC18150.16048795411901398No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17990.15907318427554057No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC17690.15642049081902795No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17060.15084983456035145No Hit
GTCTCTGGATTCACCCTTGAGGATCATGCCATGCACTGGGTCCGACAAGT16690.1475781792973192No Hit
GTATTACTGTGCAAAAGATTTCAGCGGCTTTTCGAGGGGTTTATTCGAGG16370.14474863961037238No Hit
GTGCTGTCATAGACTATGCGGACTCTGTGAAGGGCCGATTCACCATGTCC15950.1410348687712547No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15930.14085802254082053No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15590.13785163662343955No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG14750.13042409494520418No Hit
AATTGGAACAGTGCTGTCATAGACTATGCGGACTCTGTGAAGGGCCGATT14560.12874405575607953No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGA14540.12856720952564538No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14200.12556082360826437No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAAGTGTCCA14140.12503028491696186No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13410.11857539750611445No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13170.11645324274090435No Hit
CAGTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGGAGGTA13130.11609955028003602No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT13010.11503847289743095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12750.11273947190178668No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12130.10725723875832725No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12010.10619616137572219No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11590.10248239053660452No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC11370.10053708200182859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)