FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130926
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGACTATGCGGACTCTGTGAAGGGCCGATTCACCATGTCCAGAGA39010.3449385724618587No Hit
GTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGGAGGTACA30580.2703978863338538No Hit
GTCTATGACAGCACTGTTCCAATTAATACCTGAGACCCATTCCAGGCCCT26190.23158013875355238No Hit
GTCCAGAGACAGCGCCAAGAACTCCGTGTACCTCCAAATGAACAGTCTGA24080.21292286144274694No Hit
ATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGGAGGTACACGG20640.18250530980806878No Hit
TTGTATTACTGTGCAAAAGATTTCAGCGGCTTTTCGAGGGGTTTATTCGA20430.18064842438850995No Hit
CTCATGTACAGTCTCTGGATTCACCCTTGAGGATCATGCCATGCACTGGG20030.17711149977982643No Hit
GCCTTGTATTACTGTGCAAAAGATTTCAGCGGCTTTTCGAGGGGTTTATT19950.17640411485808974No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18860.1667659952994272No Hit
GTCTCTGGACATGGTGAATCGGCCCTTCACAGAGTCCGCATAGTCTATGA18770.16597018726247342No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17510.15482887474512036No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17470.15447518228425203No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17370.15359095113208115No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16420.14519075518645783No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15840.14006221450386674No Hit
GTCTGAGAGCTGAGGACACGGCCTTGTATTACTGTGCAAAAGATTTCAGC15790.13962009892778132No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15630.1382053290843079No Hit
GTCTCTGGATTCACCCTTGAGGATCATGCCATGCACTGGGTCCGACAAGT15130.13378417332345352No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14540.12856720952564538No Hit
GTATTACTGTGCAAAAGATTTCAGCGGCTTTTCGAGGGGTTTATTCGAGG14100.12467659245609351No Hit
GTGCTGTCATAGACTATGCGGACTCTGTGAAGGGCCGATTCACCATGTCC13920.12308497638218593No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13300.11760274323872649No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAAGTGTCCA13060.11548058847351639No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGA12250.10831831614093229No Hit
CAGTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGGAGGTA12010.10619616137572219No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11960.10575404579963676No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11950.10566562268441966No Hit
AATTGGAACAGTGCTGTCATAGACTATGCGGACTCTGTGAAGGGCCGATT11860.10486981464746588No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT11720.10363189103442666No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11620.10274765988225577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)