FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897539
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG40310.4491169743041807No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC34060.3794821172116198No Hit
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA33810.37669672292791734No Hit
GATTACTACTGTGCGGCATGGGATGACAGCCTGAGTGGTGTGGTCTTCGG33270.3706802712751201No Hit
ATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTG30410.3388153606695642No Hit
GCCCTATATTACTGTGCGAAAGCGGAGACTTGGGAGCCTATGGGTTGCGA30260.33714412409934275No Hit
CTATATTACTGTGCGAAAGCGGAGACTTGGGAGCCTATGGGTTGCGACTA29890.33302174055946315No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCACACCACTCAGG26570.2960317044718948No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT20990.23386170405965645No Hit
ATATTACTGTGCGAAAGCGGAGACTTGGGAGCCTATGGGTTGCGACTACT20280.22595118429394154No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19760.22015756418384047No Hit
CTTTAAAAGGTGTCCAGTGTGAGGTGCATCTGTTGGAGTCTGGGGGAGCC18920.21079863939060028No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC18750.20890457127768267No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT18340.20433652465241064No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGAAGTTATGCC17280.19252645288951234No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCAC17240.19208078980411994No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16950.18884973243502512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16890.18818123780693652No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC16310.18171912306874688No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC15980.17804240261425966No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15860.17670541335808249No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15650.17436568215977244No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14980.1669008254794499No Hit
TATGTATACTGGTACCAGCAACTCCCAGGAACGGCCCCCAAAGTCCTCAT14840.16534100468057655No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14220.1584332268569945No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14050.15653915874407684No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14030.15631632720138067No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13580.15130261749071627No Hit
GGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG13490.1502998755485834No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13370.14896288629240623No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13300.14818297589296955No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13140.1464003235514No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCACACCACTCAGGC13100.1459546604660076No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13080.1457318289233114No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA12790.1425007715542166No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12630.14071811921264704No Hit
TTACTACTGTGCGGCATGGGATGACAGCCTGAGTGGTGTGGTCTTCGGCG12590.14027245612725464No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG12580.14016104035590654No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12420.138378388014337No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12380.1379327249289446No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC12340.1374870618435522No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12240.13637290413007125No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT12150.13537016218793835No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT12060.13436742024580547No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11890.1324733521328878No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT11740.13080211556266635No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11610.1293537105351411No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT11500.128128137050312No Hit
GTGTGAGGTGCATCTGTTGGAGTCTGGGGGAGCCTTGGTCCGGCCTGGGG11380.12679114779413483No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11150.12422858505312862No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT11060.12322584311099574No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10980.12233451694021096No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC10940.12188885385481857No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG10530.11732080722954656No Hit
AGCACATACTCCGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA10460.1165408968301099No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10320.11498107603123651No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10160.11319842368966698No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10150.11308700791831885No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA10080.1123070975188822No Hit
CAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGA10030.11175001866214171No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT9980.11119293980540121No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9800.10918745592113546No Hit
CCTTTAGAAGTTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG9790.10907604014978736No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG9640.10740480357956589No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT9560.10651347740878112No Hit
GTCCTCATCTATAGGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATT9410.10484224083855967No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC9400.10473082506721157No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9350.10417374621047108No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA9090.10127693615542054No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG9060.10094268884137626No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT9020.10049702575598386No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC9010.10038560998463575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7550.077.390551
GTATCAA20000.076.103091
ATCAACG22550.067.076183
TCAACGC23200.065.196894
TATCAAC23700.063.8214262
CAACGCA24000.063.0236635
AACGCAG24600.061.4419676
GAATACG2550.059.270129
CGCAGAG26550.056.655038
GCAGAGT30200.050.0459639
ACGCAGA30450.049.635087
TACACCG700.001091155941.158315
CAGCGCT4550.041.126219
GCAGCGC5150.036.3348058
GTACATG23850.034.4799231
ACATGGG23800.033.592443
TACATGG24100.033.473152
AGAATAC4950.033.4410068
GTAATAT17850.033.137941
GGCAGCG5700.032.8288157
Produced by FastQC (version 0.11.5)