FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897539
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA32230.35909303105491797No Hit
GATTACTACTGTGCGGCATGGGATGACAGCCTGAGTGGTGTGGTCTTCGG31730.35352224248751307No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC31090.34639163312123483No Hit
GCCCTATATTACTGTGCGAAAGCGGAGACTTGGGAGCCTATGGGTTGCGA29430.3278966150774507No Hit
ATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTG26150.2913522420752747No Hit
CTATATTACTGTGCGAAAGCGGAGACTTGGGAGCCTATGGGTTGCGACTA25000.27853942837024354No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT22780.2538051271309659No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC22270.24812292279221293No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCACACCACTCAGG21490.23943249262706134No Hit
GTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG19330.21536668601587228No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17790.19820865722826528No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC17570.19575751025860716No Hit
ATATTACTGTGCGAAAGCGGAGACTTGGGAGCCTATGGGTTGCGACTACT17240.19208078980411994No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT16590.1848387646664936No Hit
CTTTAAAAGGTGTCCAGTGTGAGGTGCATCTGTTGGAGTCTGGGGGAGCC16290.18149629152605068No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16130.1797136391844811No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15280.17024329861989285No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGAAGTTATGCC14870.16567525199462085No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC14820.16511817313788038No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14510.16166428422608933No Hit
TATGTATACTGGTACCAGCAACTCCCAGGAACGGCCCCCAAAGTCCTCAT14370.160104463427216No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13970.15564783257329207No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCAC13820.15397659600307062No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC13480.1501884597772353No Hit
GGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG13250.14762589703622905No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13240.14751448126488095No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12690.14138661384073561No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12590.14027245612725464No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12370.1378213091575965No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12330.13737564607220412No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA12290.13692998298681172No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT12000.1336989256177169No Hit
TTACTACTGTGCGGCATGGGATGACAGCCTGAGTGGTGTGGTCTTCGGCG11830.13180485750479923No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA11510.1282395528216601No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11480.12790530550761584No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11470.1277938897362677No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11430.12734822665087533No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11420.12723681087952723No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11340.12634548470874246No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11280.1256769900806539No Hit
GTCCTCATCTATAGGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATT11100.12367150619638813No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT10980.12233451694021096No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT10730.11954912265650852No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10600.11810071762898325No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT10590.11798930185763516No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10550.11754363877224278No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCACACCACTCAGGC10540.11743222300089468No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT10480.11676372837280607No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10320.11498107603123651No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10160.11319842368966698No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10110.11264134483292647No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10040.11186143443348981No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9980.11119293980540121No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC9550.10640206163743302No Hit
GTGTGAGGTGCATCTGTTGGAGTCTGGGGGAGCCTTGGTCCGGCCTGGGG9510.10595639855204063No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9330.10395091466777488No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA9270.10328242003968631No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9240.10294817272564201No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC9220.1027253411829458No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG9170.10216826232620532No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9120.10161118346946484No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8990.10016277844193958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)