FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665602
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCTTCAGGTCTCAGGCTGTTCATTTGCAGATATA27690.41601437495680604No Hit
GTTTATTACTGTGCGAGAGAGGGGGGGTATCAGAGTACCAGTTCGCACTT19810.29762530761626316No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG14390.21619526383634666No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13300.19981911112045936No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12820.19260759432814203No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12320.18509559766947814No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12270.18434439800361177No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11140.1673672855550314No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10380.15594905063386227No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9980.14993945330693115No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9940.14933849357423806No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9430.14167625698240088No Hit
GTGTAGGCTCTGGATTCAGTTTCGGTGACGATGCTCTGCACTGGGTCCGC9380.14092505731653449No Hit
GATGTACACTGGTACCAGCAGTTTCCAGGAACAGTCCCCAAACTCCTCAT9350.14047433751701466No Hit
GCCTTGGGCTGACCTAGGACGGACAGCTTGGTCCCTCCGCCGAAGACCAC8800.1322111411924844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7920.11899002707323596No Hit
GATATAGTGTGTTCTTGGAATTGTCTCTGGAGATGGTGAATCGGCCGTCC7800.11718714787515663No Hit
GGTTATGATGTACACTGGTACCAGCAGTTTCCAGGAACAGTCCCCAAACT7780.11688666800881006No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7700.11568474854342385No Hit
CTCCTGTGTAGGCTCTGGATTCAGTTTCGGTGACGATGCTCTGCACTGGG7580.1138818693453445No Hit
CACTATATCTGCAAATGAACAGCCTGAGACCTGAAGACACGGCTGTTTAT7530.11313066967947813No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT7500.1126799498799583No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7430.11162827034774535No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG7400.11117755054822552No Hit
GGGCTGGAGTGGGTGGCAGTCATGTCATATGATGGAAGAGAAAAACACTA7380.11087707068187895No Hit
GTCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCC7310.10982539114966602No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT7290.10952491128331945No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7240.10877371161745308No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7240.10877371161745308No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7010.10531819315446769No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7000.10516795322129441No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG6950.10441675355542801No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCAGAGCATCGTCAC6890.10351531395638836No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA6820.1024636344241754No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6780.10186267469148229No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC6660.10005979549340296No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19350.043.9254261
CAGCCGT13650.040.6110849
CAGCGCT4450.040.4451569
GCAGCGC4650.040.25388
TCAACGC21100.039.581724
ATCAACG21150.039.4881483
TATCAAC22200.037.9447752
TAAACAG15800.037.821625
CAACGCA22250.037.5359235
AACGCAG22750.037.3439036
GGCAGCG5300.036.675367
GAGCGGT800.002107568635.9961859
CGCAGAG24650.034.4628838
ACGAGTG1801.3725185E-731.9990165
GCAGAGT26850.031.370989
ATACGAG1402.8788092E-530.8561923
TACGAGT1652.5176796E-630.5445144
AAACAGC19100.030.156146
ATAAACA19200.029.9990774
AATAAAC19350.029.3944443
Produced by FastQC (version 0.11.5)