FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665602
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCTTCAGGTCTCAGGCTGTTCATTTGCAGATATA25950.3898726265846557No Hit
GTTTATTACTGTGCGAGAGAGGGGGGGTATCAGAGTACCAGTTCGCACTT19550.29371906935375797No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16750.2516518880652402No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG13890.20868326717768276No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12240.18389367820409194No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11720.17608120167908148No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11540.1733768828819625No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10640.15985528889636752No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10370.15579881070068902No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9980.14993945330693115No Hit
GTGTAGGCTCTGGATTCAGTTTCGGTGACGATGCTCTGCACTGGGTCCGC9610.1443805757795199No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8920.13401402039056373No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8690.13055850192757834No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8550.12845514286315246No Hit
GATGTACACTGGTACCAGCAGTTTCCAGGAACAGTCCCCAAACTCCTCAT8530.1281546629968059No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8330.12514986433334035No Hit
GCCTTGGGCTGACCTAGGACGGACAGCTTGGTCCCTCCGCCGAAGACCAC8290.12454890460064723No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8270.12424842473430069No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC8190.12304650526891445No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8150.12244554553622133No Hit
CTCCTGTGTAGGCTCTGGATTCAGTTTCGGTGACGATGCTCTGCACTGGG7880.11838906734054283No Hit
GATATAGTGTGTTCTTGGAATTGTCTCTGGAGATGGTGAATCGGCCGTCC7820.11748762774150318No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT7720.1159852284097704No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7700.11568474854342385No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT7380.11087707068187895No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT7370.11072683074870569No Hit
CACTATATCTGCAAATGAACAGCCTGAGACCTGAAGACACGGCTGTTTAT7360.11057659081553241No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7220.10847323175110651No Hit
GGGCTGGAGTGGGTGGCAGTCATGTCATATGATGGAAGAGAAAAACACTA7140.1072713122857203No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC6990.10501771328812112No Hit
GGTTATGATGTACACTGGTACCAGCAGTTTCCAGGAACAGTCCCCAAACT6840.10276411429052197No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG6830.10261387435734869No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6710.10081099515926936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG957.2759576E-1268.209749
ATCAACG10950.042.748873
GTATCAA11850.042.540751
CAGCCGT11600.042.2064139
TCAACGC11100.042.1711854
CAACGCA11950.039.1715625
TATCAAC12750.037.278572
AACGCAG12750.036.705466
TAAACAG14850.035.886475
GTGCTAG2451.8189894E-1235.2728541
CGCAGAG13700.034.160198
GGCAGCG3200.031.499647
ACAGCCG21500.031.1438278
GTACATG18300.031.0886211
ATAAGAC1402.8739963E-530.8637493
TACATGG18000.030.4065072
GTACACG5300.029.893195
ACATGGG18150.029.3616543
AAACAGC17500.029.2110946
TAGCGCC1000.00628218828.7996677
Produced by FastQC (version 0.11.5)