FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178787
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT39110.33178173834628305No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGGAGTTCATTTGAAGATAGA35500.3011570368522897No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32260.2736711551790103No Hit
ATATTACTGTCAACATTATGAAACTCTCCCTCGGACTTTTGGCCAGGGGA29060.2465246053782405No Hit
ACATATTACTGTCAACATTATGAAACTCTCCCTCGGACTTTTGGCCAGGG27270.2313395040834349No Hit
ATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACCAA26210.22234720946192993No Hit
TATAAAAGGTGTCCAATGTCAAGTGCACCTGGTGGAGTCGGGGGGAGGCC22840.19375849920299426No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21250.18027005727073678No Hit
GTAATATGTTGCAATATCTTCAAGCTGCAGGCTGCTGATGGTGAAAGTAA20290.17212609233050585No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20050.17009010109544812No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG19690.16703611424286152No Hit
AGTCAGGACATTACCAAATATTTAAATTGGTATCAGCAGAAACCAGGTAA18960.16084330756956092No Hit
GTCTATTACTGTGTGAGAGCGGGGGGCGCTGCCACCGAGTCCTGGGGCCA18590.1577044877488469No Hit
TTATGAAACTCTCCCTCGGACTTTTGGCCAGGGGACCAAGTTGGAGATCA18460.15660165916319063No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC18350.15566849651378917No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18340.15558366354566178No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACC17190.14582787221101012No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16100.13658107868512293No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16080.13641141274886812No Hit
GCATGGACATGAGGGTCCCTGCTGAGCTCCTGGGGCTCCTGCTGCTCTGG16060.1362417468126133No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT15830.134290588545683No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15510.13157593356560598No Hit
CAGTAATATGTTGCAATATCTTCAAGCTGCAGGCTGCTGATGGTGAAAGT14840.12589212470106984No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14650.12428029830664912No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTGAGCTCCTGG14270.1210566455178077No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13970.11851165647398555No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13880.1177481597608389No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13680.11605150039829079No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13640.11571216852578116No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA13510.1146093399401249No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13350.1132520124500864No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13260.11248851573693976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12990.11019802559749979No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG12830.10884069810746132No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12800.10858619920307909No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA12000.10179956175288667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)