FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178787
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT37950.32194111404350406No Hit
GTCTATTACTGTGTGAGAGCGGGGGGCGCTGCCACCGAGTCCTGGGGCCA33710.2859719355574841No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGGAGTTCATTTGAAGATAGA32760.27791280358538056No Hit
ATATTACTGTCAACATTATGAAACTCTCCCTCGGACTTTTGGCCAGGGGA27640.23447832390414894No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27140.23023667549777865No Hit
ACATATTACTGTCAACATTATGAAACTCTCCCTCGGACTTTTGGCCAGGG25970.2203112182268722No Hit
ATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACCAA24740.20987676314720133No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20970.17789473416316942No Hit
TATAAAAGGTGTCCAATGTCAAGTGCACCTGGTGGAGTCGGGGGGAGGCC20470.17365308575679914No Hit
GTAATATGTTGCAATATCTTCAAGCTGCAGGCTGCTGATGGTGAAAGTAA19580.16610295159346006No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18540.15728032290820987No Hit
AGTCAGGACATTACCAAATATTTAAATTGGTATCAGCAGAAACCAGGTAA17920.15202067888431076No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17010.14430087878471684No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT17010.14430087878471684No Hit
TTATGAAACTCTCCCTCGGACTTTTGGCCAGGGGACCAAGTTGGAGATCA16490.13988956444209175No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16390.13904123476081767No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16270.13802323914328882No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15920.13505408525882964No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15180.12877644561740162No Hit
GCATGGACATGAGGGTCCCTGCTGAGCTCCTGGGGCTCCTGCTGCTCTGG15150.12852194671301942No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACC14720.12487412908354097No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14520.12317746972099286No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTGAGCTCCTGG14470.12275330488035582No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14320.12148081035844474No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13380.11350651135446863No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13160.1116401860556657No Hit
CAGTAATATGTTGCAATATCTTCAAGCTGCAGGCTGCTGATGGTGAAAGT13080.11096152231064645No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13080.11096152231064645No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA12860.10909519701184354No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12770.10833170029869689No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12690.10765303655367764No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12240.10383555298794439No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA11980.10162989581663183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)