FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925367
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34590.3737976392069309No Hit
ACTATATACACTGGGTCCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTG31790.3435393741077864No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27390.29599067180913086No Hit
GTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTT27000.29177612774175005No Hit
CTACAGACCCGTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG25670.2774034518196564No Hit
GTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT20840.22520794452363224No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19330.20889009441659362No Hit
CTATATACACTGGGTCCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGG18140.19603033174945722No Hit
GGTCTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCA17620.1904109396596161No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17600.19019480919462226No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16800.1815495905948667No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16100.17398502432008056No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15770.1704188716476814No Hit
TTATTACTGCATGCAAGCTCTACAGACCCGTCTCACTTTCGGCGGAGGGA15660.16923015409021502No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15070.1628543053728953No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14910.16112526165294416No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14310.1546413477031275No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13270.1434025635234453No Hit
TCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG12980.1402686717810339No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12870.1390799542235675No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12640.1365944538761378No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA12620.1363783234111439No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12380.13378475783121724No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12000.12967827899633336No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11990.1295702137638364No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11870.1282734309738731No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11770.1271927786489036No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11750.12697664818390975No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA11650.1258959958589403No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11560.12492340876646779No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT11290.1220056474890503No Hit
GTCTGAGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTTTCCTGCA11100.11995240807160835No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10950.11833142958415419No Hit
ATATACACTGGGTCCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGA10910.11789916865416639No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10550.1140088202842764No Hit
GCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC10540.11390075505177946No Hit
GTCTCCACTCTCCCTGCCCGTCATCCCTGGAGAGCCGGCCTCCATCTCCT10370.1120636460993314No Hit
GTCTCCACATCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCC9940.1074168411019628No Hit
GTTTATTACTGCATGCAAGCTCTACAGACCCGTCTCACTTTCGGCGGAGG9870.10666038447448417No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9750.10536360168452086No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG9680.10460714505704223No Hit
CCATGTAGACTGTGCTCGTGGACGTGTCCCAGGTCACGGTGACTCTGCCC9670.10449907982454527No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTA9550.10320229703458196No Hit
CTACTATATACACTGGGTCCGACAGGCCCCTGGACAAGGGCTTGAGTGGG9530.10298616656958808No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9440.10201357947711558No Hit
CTCCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCC9430.10190551424461862No Hit
CATGTAGACTGTGCTCGTGGACGTGTCCCAGGTCACGGTGACTCTGCCCT9400.10158131854712778No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC9320.10071679668715224No Hit
CTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT9310.10060873145465528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20450.073.770951
GGTATCA7650.066.992991
ATCAACG23600.062.9045563
TCAACGC23600.062.9045564
CAACGCA24700.060.3949055
TATCAAC25150.059.600822
AACGCAG25900.057.5250976
CGCAGAG29950.049.740878
ACGCAGA32500.046.059567
CAGCGCT3350.045.114319
GTACACG8350.035.3854145
ACACGGG9150.032.2479867
ACGGGGT9400.031.3903279
GTACATG27000.030.4770891
TATGTAC9950.030.4195732
ACTATAT17650.029.8545551
CTATATA18450.029.2948842
ACGTGCT10900.029.0906263
GAATACG2253.025525E-828.7872289
ACATGGG28200.028.6217253
Produced by FastQC (version 0.11.5)