FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925367
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30090.3251682845833059No Hit
ACTATATACACTGGGTCCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTG25210.27243245112479697No Hit
GTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTT24250.2620581888050903No Hit
CTACAGACCCGTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG24010.2594646232251636No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22310.24109353370068307No Hit
GTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT19930.2153740083664103No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17950.1939770923320153No Hit
GGTCTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCA16720.18068506873489112No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16610.17949635117742474No Hit
CTATATACACTGGGTCCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGG16510.17841569885245528No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15330.16566400141781584No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14850.16047687025796253No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14490.1565865218880725No Hit
TTATTACTGCATGCAAGCTCTACAGACCCGTCTCACTTTCGGCGGAGGGA14290.15442521723813363No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13700.1480493685208139No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13590.1468606509633475No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12590.13605412771365308No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA12570.13583799724865916No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12180.13162345318127833No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12040.13011053992632113No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11790.12740890911389752No Hit
TCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG11420.12341049551151057No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11240.12146532132656558No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10800.1167104510967No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10720.11584592923672445No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT10660.1151975378417428No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10610.11465721167925806No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA10430.11271203749431306No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10240.11065879807687112No Hit
GTCTGAGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTTTCCTGCA10130.10947008051940474No Hit
CCATGTAGACTGTGCTCGTGGACGTGTCCCAGGTCACGGTGACTCTGCCC10100.1091458848219139No Hit
GTTTATTACTGCATGCAAGCTCTACAGACCCGTCTCACTTTCGGCGGAGG9850.10644425400949029No Hit
CATGTAGACTGTGCTCGTGGACGTGTCCCAGGTCACGGTGACTCTGCCCT9680.10460714505704223No Hit
GCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC9600.10374262319706667No Hit
ATATACACTGGGTCCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGA9570.10341842749957585No Hit
GTCTCCACTCTCCCTGCCCGTCATCCCTGGAGAGCCGGCCTCCATCTCCT9550.10320229703458196No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTA9540.103094231802085No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9470.1023377751746064No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9440.10201357947711558No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9280.10028453575716446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13900.061.0806961
TCAACGC14450.058.6954044
ATCAACG14550.057.794873
CAACGCA15250.056.0936935
TATCAAC15550.054.0810852
AACGCAG16350.052.336816
GGTATCA5550.050.5601581
CGCAGAG17550.048.760868
ACGCAGA22050.038.8075647
CAGCGCT4400.037.5903789
GTACATG27000.033.8436161
ACATGGG27450.033.2527663
GAATACG2950.031.6900469
CATGGGG16550.029.098244
GCAGCGC5900.028.0335038
GTGCTAG3700.027.22471
AGCGCTG4250.027.0625065
GGCAGCG5400.026.632647
TCTACAC6600.025.0466883
CTCTAAC17300.024.9541021
Produced by FastQC (version 0.11.5)