FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974243
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCGTCGGACCGGAGCCCACTGATGGCCAGGGAGG28370.2912004499904028No Hit
GATTATTACTGTGCCGCATGGGATGACAGCCTGAGTGGTTGGGTTTTCGG24240.24880856213490887No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19320.1983078143748531No Hit
ATGCAAAGAAGGTCCAGGAAAGAGTCTCCATTAGCCGGGACACGTCCACA15810.16227984188749625No Hit
GTCTAAGACCAATCAGCGGCCCTCAGGGGTCCCTGACCGGTTCTCTGGCT15210.15612121411187968No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14510.14893614837366037No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13770.14134050745039994No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAACCACTCAGG13390.13744004319250946No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG13040.1338475103233998No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12800.1313840592131532No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12430.12758623875152297No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACTCTCAGCGTCT11890.12204347375346808No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11760.12070910440208449No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA11330.11629542116289263No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11180.11475576421898848No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACTCTCAGCGTCTGGGACCC11110.11403725764516656No Hit
ATAATAAACGGCCGTGTCCTCGGATGTCAGGCTGCTCAGCTCCATGTAGA11110.11403725764516656No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCA11080.11372932625638574No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10880.1116764503311802No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10630.10911035542467332No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10580.10859713644337193No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC10520.10798127366581027No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10360.10633897292564587No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10350.10623632912938558No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10280.10551782255556366No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10070.10336230283409786No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA9990.10254115246401566No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9980.10243850866775539No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9760.1001803451500293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)