FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974243
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCCGCATGGGATGACAGCCTGAGTGGTTGGGTTTTCGG23640.24264993435929227No Hit
GTCTAAGACCAATCAGCGGCCCTCAGGGGTCCCTGACCGGTTCTCTGGCT18510.18999366687777072No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC17240.1769579047527157No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACTCTCAGCGTCT15300.15704500827822218No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACTCTCAGCGTCTGGGACCC14310.14688327244845484No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13300.136516249026167No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG13090.1343607293047012No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13090.1343607293047012No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC12700.13035762125055045No Hit
ATGCAAAGAAGGTCCAGGAAAGAGTCTCCATTAGCCGGGACACGTCCACA12570.12902325189916683No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12340.1266624445851805No Hit
ATAATAAACGGCCGTGTCCTCGGATGTCAGGCTGCTCAGCTCCATGTAGA11120.11413990144142684No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10930.11218966931248159No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10630.10911035542467332No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10590.1086997802396322No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10550.1082892050545911No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10540.10818656125833083No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA10250.10520989116678282No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10090.10356759042661841No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9970.1023358648714951No Hit
GTGCTGACTCAGCCACTCTCAGCGTCTGGGACCCCCGGACAGAGGGTCAC9870.10130942690889234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15300.068.227031
ATCAACG16600.062.7805253
TCAACGC16700.062.4077954
CAACGCA17850.057.9933975
AACGCAG18850.055.3038836
TATCAAC19600.053.168532
CGCAGAG20900.049.5378888
GGTATCA6700.046.2033581
ACGCAGA23350.044.340127
GCAGAGT25200.041.3703089
CAGCGCT5200.035.9493689
CTTAGCG850.002856203633.83479
GTACATG29200.032.7905431
GAATACG2507.6397555E-1131.6354479
GTGCTAG3500.030.8534011
ACATGGG31850.030.0128483
CCTTACG1358.4218784E-426.6634351
CTTACGG1358.505784E-426.6182162
CATGGGG19450.025.4986274
GGCAGCG7200.024.9648427
Produced by FastQC (version 0.11.5)