FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025380
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT46480.4532953636700541No Hit
ACATTACCCCGTGGACGTTCGGCCCAGGGACCAAGGTGGAAGTCAAACGA45110.43993446332091524No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39020.38054184790029066No Hit
GGGTAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGC29810.2907214886188535No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG28600.2789209853907819No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25250.2462501706684351No Hit
GATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCATTG25020.24400709980690083No Hit
GTCCTGATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTT24620.24010610700423257No Hit
GAGTTACATTACCCCGTGGACGTTCGGCCCAGGGACCAAGGTGGAAGTCA24610.24000858218416588No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23350.22772045485576078No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22430.21874817140962377No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG21250.20724024264175234No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20860.20343677465915075No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACT19260.18783280344847764No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17450.17018081101640367No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT17360.16930308763580332No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17230.16803526497493612No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16620.16208625095086698No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA15650.1526263434043964No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14630.14267881175759228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14220.13868029413485733No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA14200.1384852444947239No Hit
ACTTACTACTGTCAACAGAGTTACATTACCCCGTGGACGTTCGGCCCAGG14090.13741247147399013No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13780.1343892020519222No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13670.13331642903118843No Hit
ATTTAAATTGGTATCAGCAGAAACCAGAGAAAGCCCCTAAGGTCCTGATC13630.1329263297509216No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG12830.12512434414558507No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC12570.12258869882385066No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTA12540.12229612436365056No Hit
CACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAAC12510.12200354990345041No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12480.12171097544325031No Hit
ATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCATTGGCAGT12460.12151592580311689No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA12060.11761493300044862No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA12010.11712730890011509No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11700.11410403947804716No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG11690.11400651465798045No Hit
GATCAGGACCTTAGGGGCTTTCTCTGGTTTCTGCTGATACCAATTTAAAT11650.11361641537771362No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC11430.11147086933624609No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGA11170.1089352240145117No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC10980.10708225243324426No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTGGGCCGAACGTC10930.10659462833291072No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA10750.10483918157171No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10700.10435155747137648No Hit
GTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTACATT10640.10376640855097623No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT10490.10230353624997562No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATT10400.10142581286937526No Hit
CCCCTAAGGTCCTGATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCA10360.10103571358910843No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10330.1007431391289083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24000.064.0374151
ATCAACG23850.063.433323
TCAACGC23900.063.3006134
CAACGCA24450.061.876675
AACGCAG26050.058.022376
TATCAAC26400.057.3062362
ACGCAGA29250.051.6721047
CGCAGAG30200.050.0466588
GCAGAGT34000.044.876579
GTACATG30350.036.3745881
TACATGG30400.035.310122
ACATGGG34250.031.3409543
TACCGTG1153.3006747E-431.2921167
CATGGGG18400.030.1480644
ATAACGA1000.006293907328.7901526
GTGCTAG4450.025.943311
CATGGGA19200.025.8901394
CTCCAAC11950.024.7173822
CAGAGTA35950.023.6235899
ATGGGGA12350.022.7501435
Produced by FastQC (version 0.11.5)