FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025380
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT44740.436326044978447No Hit
ACATTACCCCGTGGACGTTCGGCCCAGGGACCAAGGTGGAAGTCAAACGA41650.40619087557783456No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34880.3401665723926739No Hit
GGGTAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGC29370.2864303965359184No Hit
GTCCTGATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTT26360.2570754256958396No Hit
GAGTTACATTACCCCGTGGACGTTCGGCCCAGGGACCAAGGTGGAAGTCA24520.2391308588035655No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG24460.23854570988316526No Hit
GATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCATTG24120.23522986600089724No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23090.22518480953402642No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20740.20226647681835028No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19980.19485459049328055No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT18740.18276151280500885No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18060.1761298250404728No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACT18010.17564220094013927No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG16170.1576976340478652No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16150.15750258440773177No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15430.15048079736292885No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA14830.14462930815892644No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA14120.13770504593419025No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13950.13604712399305624No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13860.13516940061245586No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13300.1297080106887203No Hit
ATTTAAATTGGTATCAGCAGAAACCAGAGAAAGCCCCTAAGGTCCTGATC13230.12902533694825333No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG12290.11985800386198288No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTA12270.11966295422184946No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12050.11751740818038191No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC12000.11702978408004838No Hit
ACTTACTACTGTCAACAGAGTTACATTACCCCGTGGACGTTCGGCCCAGG11960.11663968479978155No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11550.11264116717704656No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA11520.11234859271684644No Hit
CACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAAC11380.11098324523591255No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11290.1101055218553122No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA11270.10991047221517876No Hit
ATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCATTGGCAGT11180.1090327488345784No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11110.10835007509411144No Hit
GATCAGGACCTTAGGGGCTTTCTCTGGTTTCTGCTGATACCAATTTAAAT11080.10805750063391133No Hit
GTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTACATT10870.10600947941251047No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC10780.10513175603191012No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA10520.10259611071017573No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC10420.10162086250950868No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTGGGCCGAACGTC10350.10093818876904172No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)