FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536862
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT6880.128152113578536No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA5870.10933908527703581No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC9450.032.774254
TACTGCG2653.6379788E-1232.5978247
ATCAACG9650.032.097983
ATAACGC900.003743123332.015043
GTACACG3850.031.798265
AACGCAG9750.031.748056
CAACGCA10000.030.965895
TATACTG3300.030.5512685
GGACCGA950.004892404730.3102576
TACGGAT1453.6513353E-529.7987735
GTATCAA11050.029.3432541
CGCAGAG10550.029.3406168
TACACGG4200.029.137546
AAATCCG1000.006274930728.8054815
GTATGTA5600.027.0202481
ACACGGG4550.026.896197
ACGGGGT4750.025.763729
CATGGGG7650.025.4213394
ACTGCGC3751.2732926E-1124.9554468
TATCAAC12450.024.8791582
Produced by FastQC (version 0.11.5)