FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1997587
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGGCCGGAGCCCACTGATGGCCAGGGAGG102000.510616058274308No Hit
GATTATTACTGTGCAGCATTTGATGACAGCCTGGATGCCGTGGTGTTCGG91630.4587034256830867No Hit
GTCCTGGGCCCAGTCTGTGGTGACTCAGCCACCCTCAGCGTCTGTGACCC60730.30401679626469336No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC55930.27998780528707884No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGCCACCCTCAGCGTCT53870.2696753633258526No Hit
GTAATATACGGCCGTGTCCCCAGATCTCAGGCTGCCCAACTCCAAGGACA43520.21786285153037138No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42470.21260650975401824No Hit
GACGTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCACGGCATCCAGG42260.2115552413987476No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGCCAC39540.19793881317809936No Hit
GTATATTACTGTGCGGCCTCTCTGAAAGGTAGACTTGTCCCCTACGGTTT39530.19788875278022935No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA39220.19633688044625844No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT37510.18777655241048324No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC37150.18597437808716216No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA36070.1805678551171989No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT35140.17591223811528608No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33130.16585009814341004No Hit
GCCCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAGTCTGGCACCTCAG31370.1570394681182847No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCAC31250.15643874334384436No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA30830.15433620663330308No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30160.15098215997601105No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29980.1500810728143505No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA29560.14797853610380923No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29050.1454254558124377No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG28960.14497491223160744No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC28940.14487479143586737No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28460.14247189233810592No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT27390.13711542976601268No Hit
TTATTACTGTGCAGCATTTGATGACAGCCTGGATGCCGTGGTGTTCGGCG26890.1346124098725112No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26620.13326077913002038No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT25990.13010697406420849No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG25960.1299567928705984No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25200.1261522026324761No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT24760.12394954512619474No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA24120.12074567966251283No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA23870.11949416971576206No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAAGTTTCCTGCA23850.11939404892002202No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23410.11719139141374067No Hit
GTCTGTGGTGACTCAGCCACCCTCAGCGTCTGTGACCCCCGGGCAGAGGG23380.11704121022013059No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22940.11483855271384927No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG22790.1140876467457988No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC22550.11288619719691809No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21770.10898148616305572No Hit
GTGGTGACTCAGCCACCCTCAGCGTCTGTGACCCCCGGGCAGAGGGTCAC21680.10853094258222547No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG21330.1067788286567744No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATCGAAATCATGAGCGGCCCT21180.10602792268872394No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGGTG20910.10467629194623312No Hit
ATATTACTGTGCGGCCTCTCTGAAAGGTAGACTTGTCCCCTACGGTTTGG20630.1032746008058723No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20070.1004712185251506No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA20010.10017085613793042No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29950.074.758371
GGTATCA13850.069.1350251
TCAACGC36600.060.395964
ATCAACG36750.060.149453
TATCAAC38300.057.7152022
CAACGCA39550.056.4372445
AACGCAG42450.052.2424626
CGCAGAG47100.046.1675348
ACGCAGA54150.040.5557067
TATACGG22100.036.1644065
ATACGGC23000.034.7492756
TACGGCC23250.034.6853187
GTACATG46450.033.0133931
TACATGG47000.031.099172
TATTGAG30750.030.4402945
GTAATAT26750.030.4124151
ATATACG27300.030.3309364
AATATAC28200.030.1289163
ACATGGG48950.029.8602853
GCAGAGT73100.029.3483929
Produced by FastQC (version 0.11.5)