FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654200
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAATACTGGTGACACACTCTATGCAAGACAATTCCAGGGCAGAGTCA25440.3888719046163253No Hit
GTATTAGGGATCCTGTATCCCAACCATTGAAGCCCCCGTCCGTTGGCCTG22190.33919290736777746No Hit
GCCTACATGGAGCTGACCAATCTCAGATCTGACGACACGGCCATATATTT17520.267808009782941No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14780.22592479364108836No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14740.22531335982879852No Hit
CTCTATGCAAGACAATTCCAGGGCAGAGTCACCATGACCGGGGACGCCTC14210.21721186181595842No Hit
GTCTGGGCCTGAAGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA13690.2092632222561908No Hit
GCCATATATTTCTGTGCGCGAGGCCTTGTGGAATATCCCGGCTCGTCCTC13450.20559461938245183No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12760.19504738612045247No Hit
ATATATGGCCGTGTCGTCAGATCTGAGATTGGTCAGCTCCATGTAGGCTG12580.19229593396514827No Hit
GAAATATATGGCCGTGTCGTCAGATCTGAGATTGGTCAGCTCCATGTAGG12160.18587587893610516No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA12050.18419443595230817No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12010.18358300214001833No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC11800.18037297462549678No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11780.1800672577193519No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCCTGGTCCCCCCACCGAATATCGC11690.1786915316416998No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11550.17655151329868543No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11220.17150718434729442No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11180.17089575053500458No Hit
ATATATTTCTGTGCGCGAGGCCTTGTGGAATATCCCGGCTCGTCCTCCGG10950.16738000611433812No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC10860.16600428003668602No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10790.16493427086517887No Hit
ATGCAAGACAATTCCAGGGCAGAGTCACCATGACCGGGGACGCCTCCATA10400.15897279119535312No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT10080.15408132069703454No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA10060.15377560379088964No Hit
CCATGTAGGCTGTGCTTATGGAGGCGTCCCCGGTCATGGTGACTCTGCCC10040.15346988688474472No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9680.14796698257413637No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC9380.1433812289819627No Hit
TGCATAGAGTGTGTCACCAGTATTAGGGATCCTGTATCCCAACCATTGAA9300.14215836135738305No Hit
GGATACACCTTCTCCAATTATGATATCAATTGGGTGCGACAGGCCAACGG8810.1346682971568328No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG8570.13099969428309385No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA8560.1308468358300214No Hit
ACATGGAGCTGACCAATCTCAGATCTGACGACACGGCCATATATTTCTGT8390.12824824212778965No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8300.12687251605013758No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8280.1265667991439927No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT8230.1258025068786304No Hit
AGAGAAGACCCTGTGAGCACAGCGCCTCACCATGGACTGGACCTGGAGGA8180.1250382146132681No Hit
GGATACAGGATCCCTAATACTGGTGACACACTCTATGCAAGACAATTCCA8150.12457963925405074No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8140.12442678080097828No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8100.12381534698868847No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8050.1230510547233262No Hit
CATGTAGGCTGTGCTTATGGAGGCGTCCCCGGTCATGGTGACTCTGCCCT7960.1216753286456741No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGA7910.12091103638031182No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACAAGGGTTCCCTGG7800.11922959339651482No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7740.11831244267808011No Hit
GTCTGGGTCCCGCTGATGGTCAGGGTGGCTGTGTTCCCAGAGTTGGAGCC7700.11770100886579028No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7690.11754815041271782No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGCCTCATAAGT7620.11647814124121064No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7430.113573830632834No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7290.11143381228981963No Hit
GGTCAAAGATACTCGGCCCGGAGGACGAGCCGGGATATTCCACAAGGCCT7270.11112809538367471No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA7250.1108223784775298No Hit
GCATAGAGTGTGTCACCAGTATTAGGGATCCTGTATCCCAACCATTGAAG7110.10868236013451545No Hit
GTCTTGCATAGAGTGTGTCACCAGTATTAGGGATCCTGTATCCCAACCAT7100.10852950168144299No Hit
GTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGC7100.10852950168144299No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC7100.10852950168144299No Hit
CTATTGGTCATATTTGCAGACGGTAACCGGCCCTCAACGATCCCTGAGCG6920.1057780495261388No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG6900.10547233261999388No Hit
ACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCTACAAG6680.10210944665239989No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG6670.10195658819932743No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC6660.10180372974625497No Hit
TTCCAGGGCAGAGTCACCATGACCGGGGACGCCTCCATAAGCACAGCCTA6650.10165087129318251No Hit
CTGTAGCAGCCACAGAGGTCACGGTCAGGTGGTCCTTGAACAGCGCTCTT6630.10134515438703762No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA6610.1010394374808927No Hit
GTATCAACGCAGAGTACATGGGACTCAACAACCACATCTGTCCGCTAGAG6570.10042800366860287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG14900.062.3544733
ACACCGG350.003661772261.728286
TCAACGC15450.060.130134
GTGCTAG3750.059.5744821
CAACGCA16050.057.8822755
AACGCAG16650.055.796436
GAATACG3750.055.6755949
ACGCAGA18200.051.028937
CGCAGAG18500.050.201438
GTGTAGA3650.043.4369851
TCTACAC4150.043.3866423
AGAATAC5000.043.1965838
TGCTAGA5100.042.365782
GTATAGT1502.7383066E-838.435151
TATCAAC26500.036.6903652
GTATCAA27150.036.364751
TGTAGAG5200.033.2408452
ACCGAGC900.003753797631.9974678
TTAGTAC1153.285979E-431.3138393
GGTATAC950.004881570630.3249823
Produced by FastQC (version 0.11.5)