FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654200
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAATACTGGTGACACACTCTATGCAAGACAATTCCAGGGCAGAGTCA25330.3871904616325283No Hit
GTATTAGGGATCCTGTATCCCAACCATTGAAGCCCCCGTCCGTTGGCCTG21460.32803424029348827No Hit
GCCTACATGGAGCTGACCAATCTCAGATCTGACGACACGGCCATATATTT17800.2720880464689697No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13980.21369611739529196No Hit
GTCTGGGCCTGAAGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA13920.21277896667685725No Hit
CTCTATGCAAGACAATTCCAGGGCAGAGTCACCATGACCGGGGACGCCTC13870.21201467441149496No Hit
ATATATGGCCGTGTCGTCAGATCTGAGATTGGTCAGCTCCATGTAGGCTG13180.20146744114949558No Hit
GCCATATATTTCTGTGCGCGAGGCCTTGTGGAATATCCCGGCTCGTCCTC12680.19382451849587282No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCCTGGTCCCCCCACCGAATATCGC12360.18893304799755425No Hit
GAAATATATGGCCGTGTCGTCAGATCTGAGATTGGTCAGCTCCATGTAGG11670.17838581473555487No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11660.17823295628248242No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA11640.1779272393763375No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11640.1779272393763375No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC11640.1779272393763375No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA11450.17502292876796088No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11430.17471721186181596No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11080.16936716600428003No Hit
ATGCAAGACAATTCCAGGGCAGAGTCACCATGACCGGGGACGCCTCCATA10900.16661571384897583No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10510.1606542341791501No Hit
ATATATTTCTGTGCGCGAGGCCTTGTGGAATATCCCGGCTCGTCCTCCGG10390.15881993274228065No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10270.15698563130541118No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10050.1536227453378172No Hit
CCATGTAGGCTGTGCTTATGGAGGCGTCCCCGGTCATGGTGACTCTGCCC10010.15301131152552738No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9640.14735554876184653No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9520.14552124732497707No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA9460.14460409660654233No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9250.1413940690920208No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG8890.13589116478141242No Hit
CATGTAGGCTGTGCTTATGGAGGCGTCCCCGGTCATGGTGACTCTGCCCT8830.13497401406297768No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8730.13344542953225313No Hit
GGATACACCTTCTCCAATTATGATATCAATTGGGTGCGACAGGCCAACGG8640.13206970345460103No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG8550.13069397737694893No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACAAGGGTTCCCTGG8400.12840110058086213No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC8180.1250382146132681No Hit
GTCTGGGTCCCGCTGATGGTCAGGGTGGCTGTGTTCCCAGAGTTGGAGCC8130.12427392234790585No Hit
TGCATAGAGTGTGTCACCAGTATTAGGGATCCTGTATCCCAACCATTGAA8030.12274533781718129No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGA7960.1216753286456741No Hit
ACATGGAGCTGACCAATCTCAGATCTGACGACACGGCCATATATTTCTGT7930.12121675328645674No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC7910.12091103638031182No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGCCTCATAAGT7880.12045246102109447No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC7810.11938245184958728No Hit
AGAGAAGACCCTGTGAGCACAGCGCCTCACCATGGACTGGACCTGGAGGA7560.1155609905227759No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA7480.11433812289819627No Hit
CTATTGGTCATATTTGCAGACGGTAACCGGCCCTCAACGATCCCTGAGCG7430.113573830632834No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT7380.11280953836747172No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7370.11265667991439926No Hit
GTCTTGCATAGAGTGTGTCACCAGTATTAGGGATCCTGTATCCCAACCAT7330.11204524610210945No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7260.11097523693060227No Hit
GGATACAGGATCCCTAATACTGGTGACACACTCTATGCAAGACAATTCCA7200.11005808621216753No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC7170.10959951085295015No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG7080.10822378477529807No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA6990.10684805869764598No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTC6920.1057780495261388No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6840.10455518190155914No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA6770.10348517273005198No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA6710.10256802201161724No Hit
GGTCAAAGATACTCGGCCCGGAGGACGAGCCGGGATATTCCACAAGGCCT6600.10088657902782024No Hit
GCATAGAGTGTGTCACCAGTATTAGGGATCCTGTATCCCAACCATTGAAG6580.10058086212167533No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)