FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756064
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32690.18615494651675565No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38100.074.10911
GGTATCA18300.065.338241
TCAACGC46350.060.7402654
ATCAACG47800.058.8977243
CAACGCA49300.057.2501265
AACGCAG51050.055.4286236
TATCAAC52200.054.2074972
ACGCAGA59550.047.51697
CGCAGAG63750.044.4993178
GCAGAGT74550.038.052749
CAGAGTA69900.025.7513589
GTACATG54250.025.35971
ACATGGG54900.023.4763373
TACATGG59600.022.711662
TATACGG2303.3159686E-521.9132842
GTATACG2654.0022987E-621.7447591
AGAGTAC63800.018.25924310-14
GTATAGA10600.017.6676161
CATGGGA26800.017.4633774
ATAGTAC11550.016.2084333
Produced by FastQC (version 0.11.5)