FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547662
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGA12380.22605183489086333No Hit
GTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA11670.2130876343438106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9100.1661608802509577No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG8460.15447484032122002No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC7530.13749356354831996No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA7430.13566761980929842No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA7290.13311129857466833No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATTGGGGGCCA6940.12672049548809303No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT6920.12635530674028872No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATTGGGGGCCAC6900.1259901179924844No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6870.12544233487077797No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG6830.12471195737516937No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA6660.12160785301883278No Hit
GTGTATTACTGTGCGAGAGGGGCCACCGTGGTTTACTATGATAGTAGCGC6590.12032969240151772No Hit
CATGTAGACTGTGCTCGTGGACGTGTCCCTGGTCATGGTGACTCTGCCCT6550.11959931490590912No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT6450.1177733711668876No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT6420.11722558804518116No Hit
ACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG6350.1159474274278661No Hit
CCATGTAGACTGTGCTCGTGGACGTGTCCCTGGTCATGGTGACTCTGCCC6340.11576483305396393No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT6140.11211294557592093No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA6090.11119997370641016No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC5990.10937402996738864No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAAC5710.1042613874981284No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA5670.1035310100025198No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT5660.10334841562861766No Hit
TCCTATGACAGCAGCCTGAGTGGCCCCCAATATGTCTTCGGAACTGGGAC5570.10170506626349829No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT5530.10097468876788969No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14000.053.509431
TCAACGC14150.050.878074
CAACGCA14350.050.168975
ATCAACG14400.049.9993323
AACGCAG15200.047.83716
TATCAAC16100.045.1671032
CGCAGAG17400.041.788738
ACGCAGA18500.039.6931467
GGTATCA8350.037.9569441
GGTATAT1601.9722775E-631.5139681
GTCTAAG950.004877537530.3292331
CAGCGCT1902.205361E-730.3126189
GTACATG16250.028.812771
GTACACG3103.092282E-1127.8680535
TGGACCG1607.156766E-526.9971755
GCAGAGT27300.026.8982859
ACATGGG16700.026.7301833
CAGAGTA19250.026.5530669
CTAGCAC1907.5431944E-626.525963
TATGTAC3850.026.1814692
Produced by FastQC (version 0.11.5)