FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341858
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG47590.35465749729106955No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG45480.33893303166206856No Hit
ATAATATACACCCGTGTCCTCGGCTCGCAGACTGTTCATTTGCAGAAACA45250.33721899038497366No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC40400.3010750764984074No Hit
ATATACACCCGTGTCCTCGGCTCGCAGACTGTTCATTTGCAGAAACAGCG38850.28952392876146354No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT35890.2674649627605902No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG35160.26202474479415855No Hit
GACCTAGGACGGTCAGTGTGGTCCCACCGCCGAACACCCAAGTGTTCCAC30370.22632797211031272No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT29660.22103680121145458No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG29250.21798133632619843No Hit
GGTGTATATTATTGTGCAAGAGATCTTAGGGGATCCAACTACTGGGGCCA28340.2111996947516056No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC25950.19338857017657604No Hit
GTATATTATTGTGCAAGAGATCTTAGGGGATCCAACTACTGGGGCCAGGG24840.18511645792624853No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT23410.1744595925947455No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCGGTGACGTT22650.16879580402695366No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19570.14584255562063944No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19260.14353232607325067No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG19090.14226542599887618No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG18690.1392844846474068No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTGTGGTCCCACCGCCGAACACCCA18310.1364525903635109No Hit
CTCCTGCACTGGAACCAGCGGTGACGTTGGTGGTTATAACTATGTCTCCT18270.13615449622836395No Hit
GTGTGGTCCCACCGCCGAACACCCAAGTGTTCCACCTTGTAGATGAGCTG17700.1319066548025201No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC17370.12944737818755786No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17220.12832952518075683No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17080.12728619570774255No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16980.12654096036987522No Hit
GATTAAGACTGATGGGAGTGTAACAAGCTACGCGGACGCCGTGAAGGGCC16940.12624286623472827No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC16650.12408168375491298No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC16520.12311287781568542No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC16190.12065360120072319No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTCAGTGACTACTGGATGCACTGGA16180.12057907766693644No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATCT16180.12057907766693644No Hit
GGTTATAACTATGTCTCCTGGTATCAACAACACCCAGGCAAAGCCCCCAA16130.12020645999800278No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT16120.12013193646421604No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15940.11879051285605483No Hit
CCCTGAGGAGACGGCGAGCAGGGTTCCCTGGCCCCAGTAGTTGGATCCCC15580.11610766563973236No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA15500.11551147736943848No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCT15450.11513885970050482No Hit
ACCTAGGACGGTCAGTGTGGTCCCACCGCCGAACACCCAAGTGTTCCACC15420.11491528909914463No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15260.11372291255855686No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC15170.11305220075447625No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA15070.11230696541660891No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG15040.1120833948152487No Hit
GATTATTACTGCAGCTCATCTACAAGGTGGAACACTTGGGTGTTCGGCGG14870.11081649474087422No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC14850.11066744767330075No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTG14780.1101457829367936No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14240.10612151211230995No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC14130.10530175324065585No Hit
CCCTGAGACTCTCCTGTGTAGCCTCTGGATTCACCTTCAGTGACTACTGG14040.10463104143657526No Hit
GTCTTAATCTGTGAGACCCACACCGGCCCCTTCCCTGGAGGTTGGCGGAT13860.10328961782841405No Hit
GACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCA13630.10157557655131914No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13590.10127748241617221No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13560.10105391181481199No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC13510.10068129414587833No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13440.10015962940937119No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)