FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341858
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG48440.360991997662942No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG36470.2717873277202208No Hit
ATAATATACACCCGTGTCCTCGGCTCGCAGACTGTTCATTTGCAGAAACA34970.26060879765221057No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC34960.26053427411842384No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG32190.23989125525949842No Hit
ATATACACCCGTGTCCTCGGCTCGCAGACTGTTCATTTGCAGAAACAGCG31410.23407841962413312No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT23940.17840933988544241No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC23720.17676982214213427No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT23690.17654625154077405No Hit
GACCTAGGACGGTCAGTGTGGTCCCACCGCCGAACACCCAAGTGTTCCAC23250.17326721605415774No Hit
GGTGTATATTATTGTGCAAGAGATCTTAGGGGATCCAACTACTGGGGCCA22480.1675289039525792No Hit
GTATATTATTGTGCAAGAGATCTTAGGGGATCCAACTACTGGGGCCAGGG22340.1664855744795649No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT21650.16134345064828023No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCGGTGACGTT20110.14986682644512309No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC19670.14658779095850677No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG19510.14539541441791903No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA16560.12341097195083235No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG16490.12288930721432521No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT16280.12132431300480379No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16090.11990836586285583No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTGTGGTCCCACCGCCGAACACCCA15530.1157350479707987No Hit
GTGTGGTCCCACCGCCGAACACCCAAGTGTTCCACCTTGTAGATGAGCTG15350.11439362436263749No Hit
CTCCTGCACTGGAACCAGCGGTGACGTTGGTGGTTATAACTATGTCTCCT15000.11178530068010177No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14860.11074197120708748No Hit
GATTAAGACTGATGGGAGTGTAACAAGCTACGCGGACGCCGTGAAGGGCC14490.10798460045697832No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG14160.10552532384201607No Hit
GGTTATAACTATGTCTCCTGGTATCAACAACACCCAGGCAAAGCCCCCAA14110.10515270617308241No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC13990.10425842376764159No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATCT13950.10396032963249464No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTCAGTGACTACTGGATGCACTGGA13870.10336414136220076No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA13860.10328961782841405No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13800.10284247662569364No Hit
GATTATTACTGCAGCTCATCTACAAGGTGGAACACTTGGGTGTTCGGCGG13750.10246985895675995No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC13580.10120295888238547No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC13520.10075581767966507No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG5000.046.0695089
GTCATAG21850.045.5125121
ATAGGTG21850.044.4754
TATACCG657.6004077E-444.2976045
TAGGTGG23700.042.218235
GTGGTCA24800.040.6359068
TCAACGC16400.039.5031974
GTGCTAG5750.038.8505331
ATCAACG16950.038.2213823
CAACGCA17400.037.23295
CAGAGCG17450.037.1262139
AGGTGGT27850.035.9271856
CCCCGTT7500.035.5119137
AACGCAG18050.035.49336
TCATAGG27600.035.4701842
CCGTTGA6900.035.4701849
CATAGGT28000.034.9634633
CCCGTTG7100.034.4710248
TACATGG29100.033.6418232
CTAGAGA7300.033.5266084
Produced by FastQC (version 0.11.5)