FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283348
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTACACTACCCCGTGGACTTTCGGCCAAGGGACCAAGGTGGAGATGA25000.19480296848555498No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT22850.17804991319579724No Hit
ACACTACCCCGTGGACTTTCGGCCAAGGGACCAAGGTGGAGATGAAACGA21840.17017987326898082No Hit
GTGTAACCCTGTTGACAGTAGAAAATAGCGAAATCTTCAGGTCGCAGACC18720.14586846280198357No Hit
GGGTAGTGTAACCCTGTTGACAGTAGAAAATAGCGAAATCTTCAGGTCGC16780.1307517524475045No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA16170.12599856001645696No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14630.11399869715774677No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT14500.1129857217216219No Hit
GTCTCTGGTGCCTCCATCAGTCCTCACTACTGGAATTGGTTCCGGCAGCC13390.10433646992086323No Hit
CTCCTAATCCATGCTACATCCAGTTTGCAGAGTGGGGTCCCGTCCAGGTT13180.10270012498558459No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12930.10075209530072902No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG2250.041.597735
GTATACG750.001513351338.5104331
TCAACGC36850.032.8231744
ATCAACG36800.032.672133
CAACGCA37450.032.2973025
GTACACG6800.031.7629685
AACGCAG38950.031.0535016
TACACGG7750.029.7274076
CGCAGAG44550.027.1500328
TATACTG7050.026.551745
GTATCAA47150.026.4937921
ACGCAGA47550.025.4370967
ACACGGG8800.025.362257
TTTCGCG1450.001277529424.826239
GTTACAC17400.024.826233
GTATGTA11450.024.5945431
GTTAGAC2351.414277E-624.50933
TATCAAC52500.023.6102812
GTACATG43600.023.1857551
TATGTAC10950.023.0348782
Produced by FastQC (version 0.11.5)