FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283348
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTACACTACCCCGTGGACTTTCGGCCAAGGGACCAAGGTGGAGATGA19670.15327097560443464No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19300.15038789167084846No Hit
ACACTACCCCGTGGACTTTCGGCCAAGGGACCAAGGTGGAGATGAAACGA16810.13098551600968716No Hit
GTGTAACCCTGTTGACAGTAGAAAATAGCGAAATCTTCAGGTCGCAGACC15550.12116744639801519No Hit
GGGTAGTGTAACCCTGTTGACAGTAGAAAATAGCGAAATCTTCAGGTCGC14230.11088184966197788No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT13730.10698579029226679No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA13690.1066741055426899No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC23500.030.3264084
ATCAACG24750.028.794773
CAACGCA25550.027.6114255
AACGCAG26050.027.0814556
GTACATG38050.026.3251531
TACACGG6500.025.47236
TACATGG39250.025.3100552
CGCAGAG29100.024.243028
GTACACG6850.024.1707925
TAGGACG1801.5956566E-423.9956444
GTATTAG6150.023.4350381
TACACTA18950.023.1725735
GGGTTAC18050.023.1558931
ACATGGG41350.022.8060173
GTATCAA37950.022.027081
ACGCAGA32250.021.8750977
CATGGGG20850.021.7514464
GGGTAGT14000.021.618821
TAGTGTA15400.021.502594
ACACGGG8100.021.329467
Produced by FastQC (version 0.11.5)