FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597694
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATTACTGTGCGAGAGGGGGGTGGATGTATTCCTACGGTATGGACGT25810.43182631915327907No Hit
GTAATATATAGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGG24910.4167684467302667No Hit
GTCTGGGGCTGCGGTAAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA13470.2253661572644196No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10280.17199436500951992No Hit
GCTATATATTACTGTGCGAGAGGGGGGTGGATGTATTCCTACGGTATGGA9830.1644654287980137No Hit
ATACATTGGGTGCGCCAGGCCCCCGGACAAAGGCTTGAGTGGATGGGGTG9590.16044999615187705No Hit
ATATATAGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGGCTT9300.15559801503779527No Hit
ATATTACTGTGCGAGAGGGGGGTGGATGTATTCCTACGGTATGGACGTCT8530.14271516863144018No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8340.13953628445324864No Hit
TTATTACTGCATGCAAAATACAGACTGGCCCCCCACCTTCGGCCAAGGGA8250.1380304972109474No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7390.12364186356229107No Hit
ACCAAGGACTCATCCGCGAACAAAGCCTACATGGAACTGAGCAGCCTGAG7270.12163414723922275No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA6930.11594561765719581No Hit
CAGTAATATATAGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTA6630.110926326849525No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6450.10791475236492253No Hit
ATGCTATACATTGGGTGCGCCAGGCCCCCGGACAAAGGCTTGAGTGGATG6300.10540510696108712No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG6290.10523779726749809No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA11350.043.7618185
GTATCAA11600.042.254681
GTAATAT12200.041.3582421
AACGCAG12250.039.9590346
TATCAAC12850.039.2136342
CTGTGCG13850.038.4613159
CCGCTAC1157.495677E-637.5574049
ATAGCCG12650.036.988358
ACGCAGA13050.036.957837
TAATATA14000.036.506692
TAGCCGT12850.036.412669
CGCAGAG13800.034.427628
TATATAG14800.034.0469745
ATATAGC14050.033.815036
TACTGTG16250.033.6668437
TATAGCC14350.033.6097347
ATATTAC15450.032.148653
TATATTA15800.030.9808962
TTACTGT16300.030.9138136
GAATACG1453.6619647E-529.7869079
Produced by FastQC (version 0.11.5)