FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129381
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCCCTGTACGCTTTTGGCCAGGGGACCAAGCTGGAGG60860.5388792621799021No Hit
CTATTATACTGTTGGCAGTGATAAGTTGCAAAATCATCAGGCTGCAGGCC60680.5372854687656335No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT44550.3944638700314597No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34610.306451055932409No Hit
ATTATACTGTTGGCAGTGATAAGTTGCAAAATCATCAGGCTGCAGGCCGG26120.23127713322607696No Hit
TAGTTATTCCCTGTACGCTTTTGGCCAGGGGACCAAGCTGGAGGTCAAAC22900.20276593992638445No Hit
GTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAGTATTAGCAG22300.19745329521215607No Hit
CAGTATAATAGTTATTCCCTGTACGCTTTTGGCCAGGGGACCAAGCTGGA22180.19639076626931035No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21590.19116666563365242No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21340.18895306366939058No Hit
GAATAACTATTATACTGTTGGCAGTGATAAGTTGCAAAATCATCAGGCTG21070.1865623735479878No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20740.18364041895516217No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCG20000.1770881571409471No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19730.17469746701954433No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG19540.17301512952670534No Hit
TCTCTAAGGCGTCTACTTTAGAAGATGGGGTCCCATCAAGGTTCAGCGGC19090.169030645991034No Hit
GAGTATTAGCAGTTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCC18320.16221275194110757No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18170.16088459076255046No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17940.15884807695542957No Hit
GGGAATAACTATTATACTGTTGGCAGTGATAAGTTGCAAAATCATCAGGC17810.1576970039340134No Hit
GTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGA17450.15450941710547636No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT17400.154066696712624No Hit
GTTATTCCCTGTACGCTTTTGGCCAGGGGACCAAGCTGGAGGTCAAACGA17170.1520301829055031No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16310.14441539214844237No Hit
GGTATCAGCAGAAGCCAGGGAAAGCCCCTGTGCTCCTGATCTCTAAGGCG16090.14246742241989196No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15740.13936837966992538No Hit
CTTCTAAAGTAGACGCCTTAGAGATCAGGAGCACAGGGGCTTTCCCTGGC15010.1329046619342808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14130.12511278302007914No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14130.12511278302007914No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14000.12396170999866298No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA13990.12387316592009251No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA13520.11971159422728024No Hit
GAGTTTACTCTCACCATCACCGGCCTGCAGCCTGATGATTTTGCAACTTA13280.11758653634158889No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC13190.11678963963445463No Hit
GTATTAGCAGTTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCT13010.1151958462201861No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA12650.11200825939164906No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12620.11174262715593762No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12570.11129990676308527No Hit
GTCTCTGGTGGCTCCATCAGTGATTACTACTGGAGCTGGATCCGGCAGCC12440.1101488337416691No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAAGTCAGCCTCAGGGAGA12310.10899776072025295No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12170.10775814362026631No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12130.10740396730598444No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC11980.10607580612742731No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA11620.10288821929889028No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG11490.10173714627747413No Hit
GAGTAAACTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA11420.10111733772748081No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21250.078.0148541
TCAACGC25650.063.7919854
ATCAACG26150.062.5722543
CAACGCA26950.060.7148255
TATCAAC27700.059.591372
AACGCAG28050.058.580446
CGCAGAG30950.053.2747578
ACGCAGA34700.047.337157
GCAGAGT37500.044.1575289
GAATACG1905.809852E-934.1033559
GTACATG31800.031.5062051
TAATAGT30500.030.9598674
TATACTG32850.030.9393655
TACATGG31750.030.8761272
CTATTAT30750.030.7068021
ATAATAG32050.030.1373043
TATTATA34450.029.5024132
AATAGTT31950.029.3291955
ACATGGG33500.028.8328533
GTATAAT33000.028.8315751
Produced by FastQC (version 0.11.5)