FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129381
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCCCTGTACGCTTTTGGCCAGGGGACCAAGCTGGAGG64510.5711978508581249No Hit
CTATTATACTGTTGGCAGTGATAAGTTGCAAAATCATCAGGCTGCAGGCC60780.5381709095513383No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT40900.36214528135323687No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31470.2786482152612803No Hit
ATTATACTGTTGGCAGTGATAAGTTGCAAAATCATCAGGCTGCAGGCCGG24830.21985494709048586No Hit
TAGTTATTCCCTGTACGCTTTTGGCCAGGGGACCAAGCTGGAGGTCAAAC22280.1972762070550151No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21720.19231773865506857No Hit
GAATAACTATTATACTGTTGGCAGTGATAAGTTGCAAAATCATCAGGCTG21370.18921869590510199No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20500.1815153610694708No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20290.17965593541949085No Hit
CAGTATAATAGTTATTCCCTGTACGCTTTTGGCCAGGGGACCAAGCTGGA20070.17770796569094044No Hit
GTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAGTATTAGCAG19660.17407765846955103No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19100.1691191900696045No Hit
GGGAATAACTATTATACTGTTGGCAGTGATAAGTTGCAAAATCATCAGGC18000.1593793414268524No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCG17900.15849390064114766No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17000.15052493356980504No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16990.15043638949123458No Hit
TCTCTAAGGCGTCTACTTTAGAAGATGGGGTCCCATCAAGGTTCAGCGGC16970.15025930133409365No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT16950.1500822131769527No Hit
GTTATTCCCTGTACGCTTTTGGCCAGGGGACCAAGCTGGAGGTCAAACGA16690.14778006713412037No Hit
GAGTATTAGCAGTTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCC16490.1460091855627109No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16230.1437070395198786No Hit
GTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGA15870.14051945269134156No Hit
GAGTAAACTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA15540.1375974980985159No Hit
CTTCTAAAGTAGACGCCTTAGAGATCAGGAGCACAGGGGCTTTCCCTGGC14580.12909726655575046No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA13870.12281063697724684No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA13740.12165956395583068No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13540.1198886823844212No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13500.11953450607013931No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13430.11891469752014598No Hit
GGTATCAGCAGAAGCCAGGGAAAGCCCCTGTGCTCCTGATCTCTAAGGCG13430.11891469752014598No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13220.11705527187016605No Hit
GTATTAGCAGTTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCT12800.11333642057020615No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC12660.11209680347021952No Hit
GAGTTTACTCTCACCATCACCGGCCTGCAGCCTGATGATTTTGCAACTTA12660.11209680347021952No Hit
GTCTCTGGTGGCTCCATCAGTGATTACTACTGGAGCTGGATCCGGCAGCC12420.10997174558452817No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12250.1084664962488301No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12210.10811231993454821No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12190.10793523177740727No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAAGTCAGCCTCAGGGAGA11440.10129442588462174No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11360.10058607325605796No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCATCTTCTAAAGT11330.10032044102034655No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC11320.10023189694177607No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16950.065.5517651
TCAACGC16800.064.387754
ATCAACG17250.063.128923
CAACGCA18900.057.2437175
AACGCAG19850.055.152456
TATCAAC20300.054.3569642
CGCAGAG21900.049.3320168
ACGCAGA25300.042.7024157
GCAGAGT26700.041.002859
CTATTAT31100.033.8708271
TATACTG32750.031.9341285
ATTATAC34150.031.676783
TATTATA35000.031.5270392
TTATACT33900.031.4835194
TACATGG31250.030.694562
GTACATG31750.030.6777421
ATACTGT36600.030.3054946
GTATAAT34250.030.1237371
ACATGGG31300.029.7225383
TAATAGT31700.029.5736774
Produced by FastQC (version 0.11.5)