FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912565
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19470.21335466514714022No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15650.17149463325900074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14050.15396163560951823No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10310.11297825360385289No Hit
GTACAAACTGGATGCACCATAAATCAGGAGCTTAGGGGCTTTCCCTGGTT10280.11264950989792508No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23400.066.615331
ATCAACG26500.057.5941053
TCAACGC27000.056.2609064
CAACGCA28050.054.154895
TATCAAC28800.053.023632
AACGCAG29050.052.2906886
CGCAGAG31450.048.3003048
GCAGAGT37550.040.4539159
ACGCAGA41000.037.0498667
TCGATTA1255.294383E-428.8666441
GTATACG1000.006212494328.8666441
TTTCGCG1753.986237E-628.7970529
GGTATCA23850.026.9300981
TACACCG1450.001277601724.8250455
GTAGACG2752.106317E-723.5612265
TACACGG5350.022.8761625
GTCCTAC5450.022.5106851
TACGAAT1650.002708903721.8159484
GTACATG23950.021.6951831
TACATGG25200.021.1522832
Produced by FastQC (version 0.11.5)