FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592725
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTACCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTA25400.4285292504955924No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCCTCAGGCCGCAGGCT20220.3411362773630267No Hit
ATCTATTACAGTGGCACCACCTACCACAGCCCGTCCCTCAACAGTCGAGT19720.33270066219579064No Hit
GTCTCTGGTGGCTCCATCGCCAGTGGTGGTTACTCCTGGAGCTGGATCCG16290.2748323421485512No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15750.2657218777679362No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACGGAGCTCAGCTTCAGGGAGA13450.2269180479986503No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12060.20346703783373404No Hit
ACCTACCACAGCCCGTCCCTCAACAGTCGAGTTACCATATCAGTAGACAC11490.1938504365430849No Hit
GGGTATATCTATTACAGTGGCACCACCTACCACAGCCCGTCCCTCAACAG11400.19233202581298242No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11250.1898013412628116No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10750.18136572609557552No Hit
GTTGGAGACAGCGTCACCATCACTTGTCGGGCGAGTCAGGACATTAGCAA10750.18136572609557552No Hit
GTCTATTACTGTGCCAGGTGGGGGGAGCTGGTGGGAGACTACTACTACTA10710.18069087688219662No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10540.17782276772533637No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10360.1747859462651314No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9950.1678687418279978No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9610.16213252351427726No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCCTCAGGCCGCAGGCTGC9190.15504660677379897No Hit
CTACTACGGTGTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT9130.15403433295373065No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8960.1511662237968704No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8840.14914167615673374No Hit
CAGTATAATAGTTACCCGATCACCTTCGGCCAAGGGACACGACTGGAGAT8790.1482981146400101No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8480.14306803323632375No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8420.14205575941625542No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC8340.14070606098949767No Hit
CTATTACAGTGGCACCACCTACCACAGCCCGTCCCTCAACAGTCGAGTTA8320.14036863638280822No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8310.1401999240794635No Hit
GTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGTGGCTGCCGGA8020.13530726728246656No Hit
GATCTATTCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCG7770.13108945969884853No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7590.12805263823864355No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7420.1251845290817833No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCT7290.12299126913830191No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7280.1228225568349572No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7240.12214770762157831No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7100.1197857353747522No Hit
CCGTAGTAGTAGTAGTCTCCCACCAGCTCCCCCCACCTGGCACAGTAATA7040.11877346155468388No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA7030.11860474925133915No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT6860.11573664009447888No Hit
TCCCTCAACAGTCGAGTTACCATATCAGTAGACACGTCCAAGAACCAATT6840.11539921548778945No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6780.11438694166772112No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCGCCAGTGGTGGTTACTCCTGGA6650.11219368172423974No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT6420.10831329874731115No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6290.10612003880382977No Hit
ATTCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGCAGT6260.1056139018937956No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG6100.10291450504028006No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT6070.1024083681302459No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6050.10207094352355645No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT6040.10190223122021173No Hit
AGTCAGGACATTAGCAATTCTTTAGCCTGGCTTCAGCAGAAACCAGGAAA5950.10038382049010923No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC5940.10021510818676452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4250.084.835891
GTATCAA12700.068.703721
TCAACGC12750.066.664324
ATCAACG13100.064.883223
CAACGCA13100.063.7781145
TATCAAC13850.062.4098472
AACGCAG13750.060.7170146
CGCAGAG15500.053.397548
ACGCAGA17650.047.3007937
GCAGAGT17950.046.1092959
GTACACG5400.042.681655
TACACGG5650.040.762116
ACGGGGT6200.038.306939
ACACGGG6150.037.4481167
TATGTAC6450.035.7364922
GTGTAGA3250.035.500561
CTATTAT11000.035.3997081
GTATGTA6800.034.994811
CACGGGG6600.034.8948368
GAATACG2302.910383E-1134.420729
Produced by FastQC (version 0.11.5)