FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1039183
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16100.15492940127003618No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14010.13481744793746625No Hit
ATAATATACAGCCGTGTCCTCGACTCTCAGAGAGTTCATTTGCAGATACA11840.1139356590706353No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC11630.11191484079320003No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11600.11162615246785215No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG28200.052.5901223
TCAACGC28250.052.2422034
CAACGCA29700.049.6916585
AACGCAG31000.047.607816
GTATCAA32700.046.1288451
ACGCAGA34400.042.9023867
CGCAGAG35050.042.312178
GGTATCA20700.037.655421
TATCAAC39650.037.4375422
GCAGAGT39900.036.8080949
GTACGGA2206.712071E-1032.7237746
TAGTACG3251.8189894E-1228.796924
AGTACGG2553.259629E-928.2322755
CCGTGCA3850.028.0489469
ACAGCCG8100.027.552618
GTACATG28850.026.5175911
TACATGG29650.026.247162
ACATGGG28900.025.6581573
TATACAG12700.024.9422155
CAGAGCG8300.024.286569
Produced by FastQC (version 0.11.5)