FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071431
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12250.1143330741783652No Hit
GTACAAGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTG11690.10910641935878279No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13900.052.350621
TCAACGC13350.046.9124684
ATCAACG14000.045.7627643
CAACGCA14850.043.1433535
ACGCAGA15300.041.8744287
AACGCAG15700.040.8075646
TATCAAC16500.040.574142
CGCAGAG17950.036.0934458
GGTATCA8250.033.1852381
GTACATG25800.027.6459031
GCAGAGT23950.027.0512479
TACATGG26500.026.3497282
ATAGGCG2508.3902705E-825.9150943
ACATGGG26800.024.711743
ACCGACC3501.4188117E-1024.6810428
TGCACCG4201.8189894E-1223.9954575
GCTAGAC1550.001884936923.221413
GACCGAC1952.743753E-422.1496527
ATACGAG1700.003223127221.1724623
CTTAAGC4902.0008883E-1120.5675353
Produced by FastQC (version 0.11.5)