FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418780
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG56600.3989342956624706No Hit
GTAATACACGGCCGTGTCGTCAGATTTCAGCCTGCTCAGTTCCATGTAGG46850.3302132818336881No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG37810.2664965674734631No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC37200.2621970989159701No Hit
GTGTATTACTGTGCGAAACACTCGACTGGCCTGTTTGGATCCTCTGGTTC29590.2085594665839665No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTAGGTG29070.20489434584643143No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA25880.18241023978347595No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25770.18163492578130508No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23970.1689479693821452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23950.16880700319993233No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA22550.15893937044503023No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22070.15555618207192093No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21950.15471038497864362No Hit
GTATATAGTAGTCGGTGAAGGTGTATCCAGAAGCCTTGCAGGAGACCTTC21170.14921270387234103No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20600.14519516767927373No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT20560.14491323531484798No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19810.1396270034818647No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA19410.13680767983760697No Hit
CCCTAACAGTGGTGCCACAAACTATGCCCAGAAGTTTCAGGCCTGGGTCA18700.13180338036904948No Hit
GTGTATATAGTAGTCGGTGAAGGTGTATCCAGAAGCCTTGCAGGAGACCT18660.1315214480046237No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG18180.1281382596315144No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17620.12419120652955357No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17440.12292251088963757No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17060.12024415342759272No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16700.11770676214776077No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16400.11559226941456745No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC16280.11474647232129012No Hit
CTACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGA15940.11235004722367103No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCAGATTATTCCTGCTGCTCATAT15860.1117861824948195No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15470.10903734194166818No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTAGGTGC15420.10868492648613598No Hit
CCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTG15090.10635898447962334No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGCTTATGACTATGTCTCCT14850.1046673902930687No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14350.10114323573774651No Hit
ACATGGAACTGAGCAGGCTGAAATCTGACGACACGGCCGTGTATTACTGT14340.10107275264664008No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)