FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418780
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG48780.3438165184172317No Hit
GTAATACACGGCCGTGTCGTCAGATTTCAGCCTGCTCAGTTCCATGTAGG47510.3348651658467134No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG34050.23999492521744034No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC32880.23174840355798645No Hit
GTGTATTACTGTGCGAAACACTCGACTGGCCTGTTTGGATCCTCTGGTTC26880.18945854889412028No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTAGGTG25860.18226927360126305No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23350.16457801773354572No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA22620.15943275208277535No Hit
GTATATAGTAGTCGGTGAAGGTGTATCCAGAAGCCTTGCAGGAGACCTTC22020.15520376661638874No Hit
GTGTATATAGTAGTCGGTGAAGGTGTATCCAGAAGCCTTGCAGGAGACCT20740.14618193095476395No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20730.1461114478636575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20510.14456081985931574No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19280.1358913996532232No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19020.13405883928445567No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA18880.13307207600896545No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT18490.13032323545581415No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18430.1299003369091755No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18340.1292659890892175No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17410.12271106161631826No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16530.11650854959895122No Hit
CCCTAACAGTGGTGCCACAAACTATGCCCAGAAGTTTCAGGCCTGGGTCA15940.11235004722367103No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15710.11072893612822284No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCAGATTATTCCTGCTGCTCATAT15400.10854396030392309No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15110.10649995066183623No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG14720.10375111010868493No Hit
CTGTTAGGGTTGATCCATCCCATCCACTCAAGCCCTTGTCCAGGGGCCTG14620.1030462791976205No Hit
CTACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGA14520.10234144828655606No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14220.10022695555336274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26000.077.26221
GGTATCA10550.077.119151
TCAACGC30050.064.930054
ATCAACG30200.064.607553
CAACGCA32150.060.2410055
TATCAAC32850.059.8340152
AACGCAG33100.058.51416
CGCAGAG38600.050.363138
ACGCAGA43050.044.9899337
GCAGAGT44000.043.526119
ATACACG24350.034.298884
GTAATAC26600.032.2108041
ACTATAT24750.029.6729831
GTACATG36850.029.3082581
AATACAC31000.029.2637373
TACACGG30200.028.3701065
ACATGGG37300.027.6024823
TATATAC31100.026.3915773
TAATACA32500.026.1408422
CTATATA28400.026.111982
Produced by FastQC (version 0.11.5)